摘要
目的：了解ＩＦＮγ对持续肝外性淤胆所致肝纤维化的作用。方法：制作大鼠持续肝外性淤胆模型，用药组于胆管结扎同时每日肌注ＩＦＮγ５×１０４Ｕ，用药２周；对照组则仅行胆管结扎而不用药。２周后分别测定血清生化指标、肝纤维化指标及肝能量代谢有关指标。结果：ＩＦＮγ可改善血清生化指标（Ｐ＜０．０１或Ｐ＜０．０５）。在组织学表现上，用药组肝纤维化程度显著低于单纯结扎组（Ｐ＜０．０１）。用药组大鼠淤胆后肝羟脯氨酸及血清透明质酸水平升高明显受抑（Ｐ＜０．０１）。肝ＡＴＰ及ＣＰ测定显示淤胆合并用药时两者均保持于较高水平。
Objective: To investigate the effect of IFNγ on hepatic fibrosis caused by constant cholestasis. Methods: A rat model of constant extrahepatic cholestasis was used. The treating group received IFNγ 5×10 4 units i.m. every day for two weeks after underwent bile duct ligation. The control group underwent bile duct ligation only. Serum biochemical index, hepatic fibrosis index and liver energy metabolism were detected after 2 weeks. Results:The use of IFNγ partly reversed the changes of serum biochemical index( P <0.01 or P <0.05), meanwhile improved histologic outcomes( P <0.01), reduced liver hydroxyproline and serum hyaluronic acid level( P <0.01) during cholestasis. Further determination of ATP and CP content showed IFNγ improved energy metabolism after bile duct ligation. Conclusion: IFNγ effectively suppresses hepatic fibrosis and improves liver energy metabolism during constant extrahepatic cholestasis in rats.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第1期48-50,F003,共4页
Academic Journal of Second Military Medical University
关键词
干扰素
肝外
肝纤维化
IFNΓ
胆汁淤积
interferon typeⅡ
cholestasis, extrahepatic
hepatic fibrosis
