摘要
目的:建立了中国10个省份野生与栽培地骨皮药材的指纹图谱分析方法。方法:采用高效液相色谱法,色谱条件:AichromBond-AQ C18色谱柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸水,梯度洗脱;流速:0.8 mL/min;柱温:25℃;检测波长:315 nm。结果:在选定的色谱条件下确定了12个共有峰构成14批地骨皮药材的指纹特征指纹图谱,各批样品的相似度均大于0.85。结论:该法精密度高,重现性好,可为地骨皮药材的指纹图谱评价提供依据。
AIM： To establish an HPLC-FP analysis method for identifing wild and cultivated Cortex Lycii from ten provinces in China.METHODS： The HPLC separation was performed on AichromBond-AQ C18（250 mm × 4.6 mm,5 μm） by gradient elution with a mixture consisting of methanol-0.1% phosphate acid;the flow rate was 0.8 mL.min-1;the column temperature was at 25 ℃;the detective wavelength was set up at 315 nm.RESULTS： Twelve common peaks were obtained in 14 batches of samples.The similarity of each fingerprint within one group was over 0.85.CONCLUSION： This method shows high precision,good reproducibility,and can be used for the quality assessment of Cortex Lycii.
出处
《中成药》
CAS
CSCD
北大核心
2010年第12期2032-2035,共4页
Chinese Traditional Patent Medicine
基金
河南省教育厅自然科学研究计划项目(2009A360010)
