摘要
目的研究大鼠皮层神经干细胞定向分化为神经元过程中钙调蛋白(calmodulin,CaM)与肌动蛋白(actin)的时空表达与两者的共存表达。方法采用细胞培养、免疫组化,图像分析术,免疫荧光双标技术检测神经干细胞定向分化为神经元过程中第1、3、5、7天的钙调蛋白与肌动蛋白的时空表达,共焦激光扫描显微镜(CLSM)下观察二者表达共存变化及其相关性。结果在大鼠大脑皮层神经干细胞定向分化的神经元,CaM与Actin的时空表达随着分化时间的增加,表达逐渐增强,随神经元发育逐渐成熟,CaM与Actin的阳性表达也逐渐延伸到突起,分化7天阳性表达最强。CaM的表达与Actin表达基本一致,共存表达胞体和突起。结论在大鼠大脑皮层神经干细胞定向分化的神经元CaM和actin共存表达,随着神经元分化发育成熟表达增强。
Objective To study the spatial expression and coexistence of calmodulin （CaM） and actin in neurons originated from cortical neural stem cells in rats. Methods Cell culture, immunohistochemistry, image analysis technology and immunofluorescence dual-labeling technology were applied to detect the spatial expression of CaM and action proteins at day 1, 3, 5 and 7 of cultured neurons. Confocal laser scanning microscope （CLSM） was used to observe the coexistence of CaM and action proteins. Results The spatial expression of CaM and actin was found in cell body and processes of neurons originated from cortical neural stem cells in rats with the increased density as the culture time prolonged and strongest expression at day 7 after culture. Under CLSM, coexpression of CaM and actin was seen in cell body and neurites. Conclusion CaM and actin coexpress in neurons originated from cortical neural stem cells in rats, and the expression increase as the neurons maturate.
出处
《解剖科学进展》
CAS
2010年第6期505-509,共5页
Progress of Anatomical Sciences
基金
辽宁省教育厅科学技术研究项目(No2008851
No2008779)
