摘要
目的探讨人参皂甙（ＧＳ）刺激造血祖细胞增殖有关的细胞内信号传递途径。方法采用Ｎｏｒｔｈｅｒｎ印迹杂交法、电泳带移动阻滞实验、抗体胶结合移动实验和紫外放射交联实验。结果ＮｏｒｔｈｅｒｎＢｌｏｔ显示经ＧＳ诱导的人巨核细胞株ＣＨＲＦ２８８、Ｍｅｇ０１和ＭＯ７ｅ细胞的ＧＡＴＡ２ｍＲＮＡ水平增高，分别是未经处理细胞的１．８４，２．４３和１．５２倍。但ＧＡＴＡ１ｍＲＮＡ表达水平低，且ＧＳ处理前后也无明显变化。电泳带移动阻滞实验提示ＧＳ诱导的细胞核内ＧＡＴＡ转录调控蛋白与特定的ＤＮＡ序列结合的活性增高。抗体胶结合移动实验证实与ＤＮＡ结合的主要成分为ＧＡＴＡ２蛋白，紫外放射交联实验确定该复合物的相对分子质量约为５０×１０３，符合ＧＡＴＡ２转录调控蛋白。结论ＧＳ诱导细胞内的信号传递途径与转录因子ＧＡＴＡ２有关，ＧＡＴＡ２有介导ＧＳ刺激造血细胞增殖的作用。
Objective To investigate that ginsenosides (GS) promotes
proliferation of hematopoietic cells by virtue of the intracellular signaling pathway. Methods
Three human megakaryocytic cell lines and Northern blot analysis, electrophoretic mobility
shift assay (EMSA), antibody gel supershift assay and UVradiation crosslinking were used.
Results After treatment with GS, GATA2 mRNA levels in CHRF288, Meg01 and MO7e cells were
increased by 1.84-,2.43-and 1.52- fold, respectively, while GATA1 mRNA levels remained as low
as pretreatment. GATA binding activities were elevated in all the three GS treated cell lines. The
GATA binding complex appeared as a band with an approximate molecular weight of 50000
corresponding to the size of GATA2 protein. Conclusions The intracellular signaling pathway
initiated by GS was involved in transcription factor GATA2. GATA2 might play the key role in the
GS induced upregulation of megakaryopoiesis.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1999年第6期292-295,共4页
Chinese Journal of Hematology
基金
澳大利亚政府科研基金
