摘要
从麦田和蔬菜地土样中筛选到2株具有较高抗菌活性的生防菌株YD4-6和NV11-4,测定了其抑菌活性和诱导水稻防御性相关酶活性的变化。抑菌活性测定结果表明YD4-6和NV11-4对水稻纹枯病菌、水稻稻瘟病菌、油菜菌核病菌和白菜黑斑病菌均具有较强的抑菌活性。两菌株均不产生几丁质酶活性,但NV11-4能产生纤维素酶活性。针对其对水稻病原菌的抑菌活性和纤维素酶活性的差异及其特性,研究了2个菌株诱导水稻防御性酶活性的变化。结果表明,YD4-6和NV11-4菌株均可有效诱导水稻PPO、POD、PAL、SOD活性增强,MDA含量升高。接种水稻纹枯病菌和使用YD4-6和NV11-4菌株,在使用48 h后,水稻防御酶的活性增加并达到最高,其中NV11-4菌株诱导活性比较持久;YD4-6使用后,诱导水稻的MDA含量增幅较大。结果显示,2个菌株均可有效的诱导水稻防御性酶活性增强和MDA含量增加。经16S rRNA鉴定后,菌株Y4-6确定为蜡质芽孢杆菌,NV11-4确定为枯草芽孢杆菌。
YD4-6 and NV11-4 strains were isolated from wheat soil and vegetable soil,both of them exhibited a better antifungal activities to several plant pathogens.The results exhibited that YD4-6 and NV11-4 showed antifungal activities to Rhizoctonia solani,Magnaporthe grisease,Sclerotinia scle-rotiorum and Alternaria brassicae,while NV11-4 also can inhibit the growth of Fusarium moniliforme.Both of these two isolates exhibited without chitinase activity,NV11-4 with cellulase activities.Based on these differences to antifungal and cellulase activities of the YD4-6 and NV11-4,their effects on rice defense enzyme were observed.The results indicated that YD4-6 and NV11-4 effectively increased rice PPO,POD,PAL and SOD activities,also promoted concentration of MDA.When the R.solani used with isolates YD4-6,NV11-4,respectively,the induced activities of PPO,POD,PAL,SOD and con-centration of MDA were higher than that of only used R.solani,YD4-6 or NV11-4.Compared with two antagonistic bacteria induced activities to rice defense enzyme,NV11-4 exhibited more durable than that of YD4-6,also YD4-6 induced accumulation of MDA,these difference maybe related with charac-ter of these two isolates.These two isolates were identified in database by using 16S rRNA sequence of YD4-6 or NV11-4.YD4-6 identified as Bacillus cerecus,and NV11-4 as Bacillus subtilis.
出处
《微生物学通报》
CAS
CSCD
北大核心
2010年第12期1753-1759,共7页
Microbiology China
基金
江苏省农科院院基金人才项目(No.6510803)
江苏省农业科技自主创新项目[No.cx(08)120]
江苏省自然科学基金项目
