摘要
目的:为了解猪链球菌2型强毒株05Z33转录调控因子Rgg的调控作用,用基因芯片方法分析野生株与rgg基因敲除突变体之间的差异表达基因。方法:用猪链球菌2型全基因组序列点样制备芯片,将芯片运用于rgg敲除株与野生株的基因表达差异研究,采用定量real-time PCR(qRT-PCR)验证表达谱结果。结果:在突变体中共发现45个基因表达量变化在2倍以上,其中19个基因表达上调,26个基因表达下调。这些基因在细菌毒力、免疫抗原、DNA合成和修复、基础代谢和ABC转运系统等方面起着重要作用。结论:转录调控因子Rgg是一个全局调控因子,但rgg敲除后并不影响猪链球菌的毒力。
Objective： To understand the role of transcriptional regulator Rgg of virulent Streptococcus suis type 2 05Z33,gene microarray method was used to analyze differentially expressed genes between the wild strain and the rgg gene knock-out mutant.Methods： The whole genomic sequences of S.suis type 2 were used to print onto glass slides;the microarray was then applied to differential expression analysis between rgg knock-out strain and wild strain;the results were validated with quantitative real time-PCR（qRT-PCR）.Results： The expressions of 45 genes of the mutant were found to change above two times,of which 19 genes were upregulated,26 genes were down-regulated.These genes play an important role in bacterial virulence,immune antigen,DNA synthesis and repair,basic metabolism and other ABC transporter systems.Conclusion： The transcription regulator Rgg is a global regulatory factor,while the virulence of S.suis is not affected after the rgg knock-out.
出处
《生物技术通讯》
CAS
2010年第6期812-817,共6页
Letters in Biotechnology
关键词
猪链球菌2型
基因芯片
转录调控因子
Streptococcus suis type 2
microarray
transcriptional regulator
