摘要
目的:研制猪链球菌2型(SS2)全基因组DNA芯片,建立SS2基因表达谱技术平台。方法:利用SS2全基因组序列,挑选出2194条基因,经PCR扩增出2156条基因并将产物纯化,点样制备芯片;将芯片用于表达谱研究,采用实时定量PCR验证表达谱结果,对芯片进行可靠性分析。结果:芯片杂交数据与实时定量PCR验证显示了较高的相关性,二者相关系数r=0.87。结论:研制了一批SS2全基因组DNA芯片,并建立了基于DNA芯片的表达谱技术平台。
Objective： To develop a whole-genome DNA microarray of Streptococcus suis serotype 2（SS2） and build up a method of gene expression profiling. Methods： Based on the genomic sequences of SS2, a total number of 2 194 genes were chosen, 2 156 of those were successfully amplified by PCR and purified, the PCR products were finally printed onto glass slides; the microarray were applied to gene expression profiling and the results were compared and validated with quantitative real time-PCR（qRT-PCR）. Results： Microarray hybridization result was mainly consistent with theory expectations and PCR verification results. The comparison of hybridization and qRT-PCR results showed a positive correlation（r= 0.87）. Conclusion： We developed a batch of whole-genome DNA microarray of SS2 with good quality, established a platform of microarray-based gene expression profiling of SS2, and built up a set of microarray data analysis standard methods.
出处
《生物技术通讯》
CAS
2009年第5期658-661,共4页
Letters in Biotechnology
基金
国家自然科学基金(30600023)
关键词
猪链球菌2型
DNA芯片
芯片杂交
基因表达谱
Streptococcus suis Serotype 2
DNA microarray
microarray hybridization
gene expression profiling
