摘要
目的 观察胰岛素样生长因子干预对心力衰竭大鼠心肌细胞黏附分子-1（ICAM-1）表达的影响. 方法 应用异丙肾上腺素腹腔注射方法建立SD大鼠心力衰竭模型.大鼠随机分为对照组、心力衰竭组、血管紧张素转换酶抑制剂（ACEI）治疗组和胰岛素样生长因子-1（IGF-1）治疗组.应用生理记录仪检测大鼠血流动力学改变;分别应用放射免疫法和免疫组化法测定血管紧张素Ⅱ含量和大鼠心肌ICAM-1表达的改变. 结果 与模型组比较,ACEI和IGF-1改善心力衰竭大鼠血流动力学,降低异丙肾上腺素AngⅡ含量,抑制ICAM-1的表达.与ACEI组比较,IGF-1改善心力衰竭大鼠的血流动力学参数更加明显,另外,IGF-1降低AngⅡ的浓度（369.2±63.0）ng/L与（384.4±56.2）ng/L使ICAM-1（0.804±0.024与1.254±0.059）表达更加显著（P＜0.01）. 结论 IGF-1可明显抑制心力衰竭大鼠心肌ICAM-1的表达,这可能与其抑制肾素-血管紧张素-醛固酮系统系统激活、降低AngⅡ有关.
Objective To investigate the effect of insulin-like growth factor-1 （IGF-1） on intercellular adhesion molecule-1 （ICAM-1） expression in rat model with congestive heart failure （CHF）. Methods The model of CHF in male Sprague-Dawley rat was established using subcutaneous injection of isoproterenol. The rats were randomized into model group （n = 10）,angiotensin converting enzyme inhibitors （ACEI） group （ n=10） and IGF- 1 group （ n = 10）. The rats injected with saline were used as normal controls （n=10）. The haemodynamic parameters of rats in each group were detected. The concentration of plasma angiotensin Ⅱ （Ang Ⅱ ） and expression of ICAM-1 in rat myocardium were determined by radioimmunoassay and immunohistochemistry,respectively. Results Compared with model group, ACEI and IGF-1 could rescue the diversities of hemodynamic parameters. In addition, ACEI and IGF-1 could also significantly down-regulated concentration of plasma Ang Ⅱ and inhibited ICAM-1 expression. Compared with ACEI, IGF-1 more significantly inhibited ICAM-I expression （0. 804 ± 0. 024 vs. 1. 254 ± 0. 059） and down-regulated concentration of plasma AngⅡ [（369.2±65.0） μg/L vs. （384.4±56.2） μg/L]. Conclusions IGF-1 can suppress ICAM-1 expression in rat model with CHF induced by isoproterenol. This effect may be related to inhibiting activation of RAS system.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2010年第11期940-943,共4页
Chinese Journal of Geriatrics
