摘要
目的了解2008年医院革兰阴性菌的临床分布及耐药性。方法菌株鉴定采用法国生物梅里埃公司的VITEK 60分析仪,药敏试验采用K-B法,纸片扩散确证试验检测ESBLs。结果前10位细菌构成比依次是大肠埃希菌(27.1%)、肺炎克雷伯菌(15.4%)、副流感嗜血菌(8.7%)、流感嗜血菌(7.4%)、阴道加德纳菌(6.5%)、鲍氏不动杆菌(5.4%)、铜绿假单胞菌(4.7%)、淋病奈瑟球菌(4.4%)、奇异变形菌(3.7%)、阴沟肠杆菌(2.6%);大肠埃希菌中超广谱β-内酰胺酶(ESBLs)菌株占55.7%、肺炎克雷伯菌中ESBLs占55.0%。结论对产ESBLs的肺炎克雷伯菌和大肠埃希菌、耐亚胺培南鲍氏不动杆菌和铜绿假单胞菌,应加强隔离预防,控制在医院内的扩散,减少耐药菌株产生。
OBJECTIVE To investigate the clinical Gram-negative bacteria distribution and drug resistance in our hospital in 2008.METHODS The clinical isolates were identified by VITEK-60 of Bio-Merieux of France.Using K-B agar diffusion method and confirmed test to detect the drug resistance of ESBLs-producing Klebsiella pneumoniae and Escherichia coli.RESULTS The top 10 bacteria were E.coli(27.1%),K.pneumoniae(15.4%),Haemophilus parainfluenzae(8.7%),H.influenzae(7.4%),Gardnerella vaginalis(6.5%),Acinetobacter baumannii(5.4%),Pseudomonas aeruginosa(4.7%),Neisseria gonorrhoeae(4.3%),Proteus mirabilis(3.8%),and Enterobacter cloacae(2.6%).The extended-spectrum β-lactamases(ESBLs) producing strains of E.coli accounted for 55.7%,and that of K.pneumoniae for 55.0%.CONCLUSION We should strengthen the isolation,prevention and control of spreading of ESBLs-producing K.pneumoniae and E.coli,and imipenem-resistant A.baumannii and P.aeruginosa in hospitals.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2010年第23期3757-3759,共3页
Chinese Journal of Nosocomiology
关键词
革兰阴性菌
耐药性
临床分析
Gram-negative bacteria
Resistance
Clinical distribution