摘要
目的:探讨DLX4在妊娠期高血压疾病中的作用及其对滋养细胞EMT的影响。方法:采用Western blot法分别检测子痫前期胎盘组织及缺氧培养的滋养细胞系JEG-3中DLX4蛋白表达的变化;通过实时PCR技术,比较缺氧条件下滋养细胞中DLX4、HIF-1α和E-cad表达变化的差异。结果:滋养细胞缺氧培养后DLX4蛋白表达明显下降(P<0.05),与其在子痫前期胎盘组织的表达变化一致;缺氧抑制滋养细胞中DLX4 mR-NA表达,同时伴有HIF-1α和E-cad mRNA表达增加。结论:缺氧引起滋养细胞DLX4表达下调,伴随EMT相关因子表达的改变。子痫前期滋养细胞侵袭力异常可能与缺氧引起DLX4表达下调及滋养细胞EMT异常有关。
Objective：To investigate whether DLX4 expression levels are altered in preeclampsic placenta and hypoxia-treated JEG-3 cells,and explore its relationship with EMT of trophoblast.Methods：The expression of DLX4 in preeclampsic placenta and hypoxia-treated JEG-3 cells was determined by Western Blot.Real time PCR was used to study the different mRNA expression of DLX4,HIF-1α and E-cad in JEG-3 cells cultured under hypoxia conditions.Results：The expression of DLX4 protein was significantly decreased in hypoxia-treated JEG-3 cells（P0.05）.The changes were corresponding to its expression in preeclampsic placenta.Hypoxia inhibit expression of DLX4 mRNA in JEG-3,while the expression of HIF-1α、E-cad mRNA were increased（P0.05）.Conclusion：Hypoxia induces the downregulated expression of DLX4,and the alteration of EMT in trophoblast,which maybe affect the invasive capacity of trophoblast in preeclampsia.
出处
《现代妇产科进展》
CSCD
北大核心
2010年第11期810-812,共3页
Progress in Obstetrics and Gynecology
基金
国家自然科学基金项目(No:30600674)
关键词
缺氧
DLX4
子痫前期
上皮细胞间质化
滋养层
Hypoxia
DLX4
Preeclampsia
Epithelial mesenchymal transition
Trophoblast
