摘要
对唾液乳杆菌代谢低聚果糖的代谢过程进行探讨,采用阴离子交换色谱检测唾液乳杆菌代谢低聚果糖时各组分的变化规律,采用高效液相色谱方法检测其代谢产物。结果表明:唾液乳杆菌能够代谢低聚果糖,发酵液OD600值达到0.303时,总糖质量浓度从最初的10.00g/L降至8.82g/L,pH值从7.00降至6.12。在与低聚果糖组分同时存在的情况下,唾液乳杆菌优先利用葡萄糖、果糖和蔗糖;低聚果糖组分中能快速利用蔗果三糖,蔗果四糖代谢效率不高,不能代谢蔗果五糖。代谢产物HPLC分析结果显示,唾液乳杆菌代谢低聚果糖的代谢产物主要有乳酸和乙酸。
The metabolism of fnmctooligosaccharides （FOS） by Lactobacillus salivairus FD89 was discussed. Changcs in composition and metabolites of FOS were detected by anion-exchange chromatography and HPLC, respectively. The results showed that FOS could be metabolized by L. salivairus FD89. The concentration of total sugar reduced from 10.00g/L to 8.82g/L when OD600 reached 0.303 and pH value reduced from 7.00 to 6.12. Glucose, fructose and sucrose were prior to be metabolized when they existed with FOS. Kestose was metabolized rapidly, while nystose was con- sumed inefficiently. 1F-Fructoffanosylnystose was hardly used by L. salivairus FD89. The results of metabolites analysised by HPLC showed that the main products of FOS were lactic acid and acetic acid.
出处
《中国酿造》
CAS
北大核心
2010年第12期114-117,共4页
China Brewing
关键词
低聚果糖
唾液乳杆菌
代谢
fructooligosaccharides
Lactobacillus salivairus
metabolism
