摘要
从舟山海域海水中筛选到一株海洋细菌NF19,经16S rDNA分子鉴定和API鉴定结果显示菌株NF19为唾液乳杆菌属(L.salivarius)的一个种,将序列上传至NCBI获得GenBank登录号为FJ405036。通过对菌株NF19进行发酵条件优化及正交试验,得到菌株NF19的最佳发酵条件为:初始pH7.2,接种量为8%,在120 r/min下20℃培养48 h。此条件下发酵液有最佳抑菌效果。
Marine bacteria strain NF19 was isolated from ZhouShan Sea,16S rDNA sequences analyses and API showed the strain belongs to Lactobacillus salivarius.Submitting 16S rDNA sequence to NCBI and getting GenBank accession FJ405036.Through culture condition and orthogonal tests,we obtained an optimal fermental condition： initial pH 7.2,inoculation level 8%,120 r/min shake revolutions,culture temperature was 20 ℃ and culture time was 48 hours.The effect of inhibition was best.
基金
浙江省教育厅计划项目(20061133)
舟山市科技局计划项目(06226)
校级课题(X05LQ02)
关键词
发酵条件
唾液乳杆菌
抑菌
fermentation
Lactobacillus saliva
inhibition
