摘要
目的观察多柔比星肾病大鼠肾脏转化生长因子β1(TGF-β1)的表达及过氧化物酶体增殖激活受体γ(PPARγ)激动药——罗格列酮对TGF-β1表达的影响,探讨罗格列酮对肾脏的保护作用。方法将实验大鼠30只随机分为正常组、多柔比星肾病模型组、治疗组各10只,观察检测各组24h蛋白尿,血液生化指标(包括肾功能、血脂、血清蛋白),肾脏病理改变;免疫组化方法检测TGF-β1、PPARγ表达,并进行相关分析。结果模型组表现为高脂血症、低蛋白血症及大量蛋白尿;治疗组24h尿蛋白定量和血脂降低,血浆蛋白升高,与模型组比较差异有显著性,肾组织病理损害明显减轻,PPARγ表达上调,TGF-β1表达显著低于模型组。结论罗格列酮通过上调PPARγ表达,降低肾脏TGF-β1,具有抗纤维化肾脏保护作用。
Objective To investigate the protective effect of peroxisome proliferators-activated receptor gamma（PPARγ）agonist rosiglitazone（RSG）against doxorubicin-induced nephropathy in rats by regulating transforming growth factor beta1（TGF-β1）expression.Methods Thirty rats were randomly divided into the normal group,model group and RSG-treated group,ten in each.Total serum protein,serum albumin,serum creatinine,urea nitrogen,blood glucose,cholesterol,triglyceride and urine protein within 24-hour were detected.Results By the end of the experiment,hyperlipemia,hypoproteinemia and hyperproteinuria were observed in the model group.There were significant differences in TP,ALB,TC,TG and 24 hour urine protein between the model group and RSG-treated group,and nephrotic pathological lesion was ameliorated in RSG-treated group with increased expression of PPARγ and decreased expression of TGF-β1.Conclusion RSG has anti-fibrosis and protective effect on kidney by upregulating the expression of PPARγ and downgrading that of TGF-β1.
出处
《医药导报》
CAS
2010年第12期1552-1555,共4页
Herald of Medicine
关键词
罗格列酮
肾病
多柔比星
转化生长因子β1
肾纤维化
Rosiglitazone
Doxorubicin-induced nephropathy
Transforming growth factor beta1
Renal fibrosis
