摘要
目的:观察新型胰岛素增敏剂罗格列酮对单侧输尿管梗阻(UUO)的肾间质损伤的抗氧化保护作用。方法:将雄性SD大鼠随机分成三大组:假手术对照组(Sham组,n=6)、UUO模型组(结扎左侧输尿管制作UUO模型,n=18)、UUO罗格列酮干预组(UUO+RSG组,n=18)。除Sham组外,其余2组根据不同的观察时间细分为3、71、4 d亚组(,n=6)。分别测定大鼠左肾皮质匀浆中脂质过氧化物标志物MDA以及抗氧化酶CAT、SOD含量。结果:与Sham组相比,UUO模型组大鼠梗阻侧肾皮质在3、7、14 d时MDA含量分别升高了8.9(P=0.000)、3.5(P=0.000)和0.7倍,而CAT含量分别下降了66.0%、72.6%和83.8%(P=0.000),SOD分别下降了42.0%、50.2%和67.7%(P<0.005)。与UUO模型组相比,UUO+RSG组大鼠梗阻侧皮质MDA含量在3、7 d时分别减少了80.0%和65.3%(P=0.000),14 d时差异不显著;在3、71、4 d时CAT分别增加了96.2%、92.1%和102.9%(P<0.005),SOD分别增加了33.3%、35.1%和55.4%(P<0.005)。结论:RSG能减少单侧输尿管梗阻侧肾皮质脂质过氧化物的产生,同时增加抗氧化酶的含量,从而改善单侧输尿管梗阻大鼠氧化应激。
Objective：To investigate the antioxidative effect of rosiglitazone（RSG）, a new insulin sensitizer, on kidney of rats with unilateral ureteral obstruction （UUO）. Methods： Male adult Sprague-Dawley rats were randomly divided into 3 groups： Sham-operated group （n = 6）, UUO model group （UUO model was established by ligating the left ureter, n = 18）, and UUO＋RSG group （UUO model rats were treated with RSG,n= 18）. The latter 2 groups were divided into subgroups according to the times （3 d, 7 d and 14 d） after intervention （n=6）. Malondialdehyde （MDA）, catalase （CAT） and superoxide dismutase （SOD） contents in the homogenized lysate of obstructive renal cortex were determined. Results： Compared with those in Sham-operated group, MDA contents in UUO group increased by 8.9 （P=0. 000）, 3.5 （P=0. 000） and 0.7 times on d 3, d 7 and d 14, respectively, while the contents of CAT and SOD decreased dramatically, with CAT contents reduced by 66.0%, 72.6% and 83.8%, respectively （P=0. 000） and SOD contents reduced by 42.0%, 50.2% and 67.7%, respectively（P〈0. 005）. Compared with those in UUO model group, MDA contents in UUO＋RSG group decreased by 80.0% and 65.3% on d 3 and d 7（P=0. 000）, respectively; no significant difference was found between the 2 contents on d 14; CAT contents were increased by 96.2%, 92.1 % and 102.9%, respectively （P〈0. 005） and SOD content were increased by 33.3%, 35.1% and 55.4 % （P〈0. 005）, respectively. Conclusion： Rosiglitazone can suppress oxidative stress in rats with UUO by reducing lipid peroxidation production and improving antioxidative enzyme contents.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第2期192-195,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30200125)
霍英东基金(81038)
上海市卫生局科委基金(03JC14084)~~
