摘要
以6-巯基烟酸(6-MNA)为修饰剂,在水溶液中合成CdSe纳米晶,考察了pH值、反应物不同配比、反应温度及反应时间等条件对合成CdSe纳米晶荧光强度的影响,获得最佳合成条件为:反应物配比Cd2+∶HSe-∶6-MNA=1∶0.5∶3,溶液pH值为9.70,回流温度60℃,回流时间30min。透射电镜观察所合成的纳米晶形貌为树状,与传统的球状不同,激发及发射波长分别位于400nm和543nm,与传统的球状纳米晶相似。同时考察了缓冲溶液的体积与pH值、反应温度、反应时间对体系荧光强度的影响。在最佳实验条件下,体系的荧光强度与牛血清白蛋白(BSA)的浓度呈线性关系,线性范围为0～4.5×10-7mol/L,相关系数R=0.9851,检出限为1.0×10-8mol/L。该方法用于合成样及实际样品的测定,结果令人满意。
Water-soluble 6-mercapto-nicotinic acid capped CdSe nanoparticles were synthesized.The reaction conditions such as pH value,molar ratio of reactants,reaction time and temperature were optimized.The transmission electron microscopy results showed that the synthesized CdSe nanoparticles were dendroides-like.The excitation and emission wavelengths were 400nm and 543nm,respectively. The influence on the fluorescence intensity of CdSe/6-MNA in the presence of bovine serum albumin （BSA）was studied.It was found that the relative fluorescence intensity was linearly proportional to the concentration of BSA in the range of 0～4.5×10^-7 mol/L with a detection limit of 1.0×10^-8 mol/L. The method was applied for the analysis of milk samples with satisfactory results.
出处
《分析科学学报》
CAS
CSCD
北大核心
2010年第6期626-630,共5页
Journal of Analytical Science
基金
国家自然科学基金(No.20975041)
广东省自然科学基金重点项目(No.10251063101000000)
