摘要
将异硫氰酸荧光素(FITC)与3-氨基丙基三甲氧基硅烷(APTMS)反应制得前驱体FITC-APTMS,采用油包水微乳液法,利用APTMS与正硅酸乙酯(TEOS)的共水解与聚合作用,制备了FITC掺杂的二氧化硅核壳型荧光纳米粒子。经TEM与荧光光谱表征及光稳定性实验与染料泄露实验等,表明所制得纳米颗粒呈规则球形,粒径为(150±15)nm,具有良好的单分散性与光稳定性,不易发生染料泄露。这种纳米颗粒对pH值敏感,在pH3.6～9.7范围内,荧光强度与溶液酸度有良好的响应,其中在pH6.0～9.0之间呈良好的线性关系,此纳米颗粒能被单个小鼠神经干细胞吞噬,可应用于细胞的pH值实时监测。
A novel core-shell fluorescein isothiocyanate（FITC） doped silica fluorescent nanoparticle was developed with a water-in-oil（W/O） microemulsion method.To prepare the nanoparticle,a silane reagent of 3-aminopropyl-trimethoxysilane（APTMS） was first used to synthesize a novel precursor of FITC-APTMS.By characterizations using transmission electron microscopy and fluorescent spectra,photostable experiment and dye leaking experiments,it was found that the nanoparticles are spherical and uniform in size of 150±15 nm with monodisperse,high fluorescent signal and high phtostability.No obvious dye leaking was observed.The possibility of using the fluorescent nanoparticles as a novel pH probe was also investigated.It was found that the fluorescence nanoparticles are pH sensible.The pH response rang and pH responsive linear ranges are 3.6-9.7 and 6.0-9.0,respectively.The nanoparticles could be phagocytosed by single murine neural stem cell and could be applied to detect pH value for this single alive cell.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第4期488-492,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.40830744)
上海市重点学科项目(No.S30109)
上海市浦江人才计划(D类)
上海市纳米专项项目(No.0752nm024)资助
