摘要
目的:观察张氏益胃汤对胃溃疡大鼠生长因子VEGF、bFGF、EGF及TGF-a表达的影响,探讨张氏益胃汤治疗胃溃疡的作用机制。方法:将50只SD大鼠随机分为正常对照组、溃疡模型组、中药小剂量组、中药大剂量组、西药治疗组共5组,每组10只。采用改良Okbae乙酸涂抹法复制实验性胃溃疡模型,中药治疗组给予张氏益胃汤灌胃,西药治疗组给予雷尼替丁灌胃,共14d;实验后ELISA检测各组血清VEGF和bFGF以及免疫组化检测胃黏膜组织EGF和TGF-a含量的变化。结果:中药治疗组和西药治疗组均能较正常对照组明显增加大鼠胃溃疡血清VEGF、bFGF和胃组织EGF、TGF-a水平,差异具有统计学意义(P<0.05,P<0.01)。结论:张氏益胃汤通过促进细胞生长因子VEGF、bFGF、EGF、TGF-a的表达,从而发挥抗溃疡作用。
Objective：To study the effect of ZhangSiYiWei decoction on serum levels of EGF,VEGF,FGFand TGF-a in rats with gastric ulcers.Methods：The gastric ulcer model was established by dropping acetic acid on the stomach surface.The rats were randomly divided into different groups：the normal control group,the model group,the herb group and the group of western medicine.All of these rats were treated with different medicines for 14 days.Then,the quantitative detection was done to see the changes of content of EGF,VEGF,bFGF and TGF-a in the gastric mucous.Results：Compared with the normal control group,EGF,VEGF,bFGF and TGF-a content in ZhangSiYiWei decoction group increased significantly（P〈0.05,P〈0.01）.Conclusion：ZhangSiYiWei decoction promotes the healing of damaged mucosa through regulating the content of EGF,VEGF,bFGF and TGF-a,which is possibly part of the mechanism in healing the gastric mucosa.
出处
《江西中医学院学报》
2010年第5期64-66,共3页
Journal of Jiangxi College of Traditional Chinese Medicine
基金
江西省教育厅科学技术研究项目(GJJ09283)
江西省卫生厅中医药科研项目(2008A056)
关键词
张氏益胃汤
胃溃疡
生长因子
ZhangSiYiWei decoction
Gastric ulcer
Growth factor
