摘要
使用荧光猝灭法测定植物液泡膜Ｈ＋ＡＴＰａｓｅ质子转运活性．比较了两种常用荧光染料吖啶橙和喹亚因在不同浓度的测定灵敏度．探讨了不同蛋白量和缓冲系统对测定结果的影响．得到了用５μｍｏｌ／Ｌ吖啶橙，２００～２５０μｇ蛋白质含量，ＨｅｐｅｓＴｒｉｓ（ｐＨ７０）为缓冲介质。
Fluorescence quenching method was to measure the proton pumping activity of tonoplast H + ATPase from higher plants. The sensitivity of two common fluorescent dyes was compared. Acridine orange is better than quinacrine with the initial slope and maximum quenching. The optimim for the fluorescence measurement of acridine orange dye were: 5 μmol/L acridine orange, 200～250 μg protein, Hepes Tris of Hepes BTP(pH 7 0). Initiating with ATP or Mg 2+ was both valid.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第2期184-187,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
关键词
液泡膜
H^+-ATPASE
泵活性
荧光猝灭法
tonoplast membrane, H + ATPase, pump activity, fluorescence quenching method
