摘要
目的:建立测定保健食品中大豆低聚糖(包括蔗糖、棉子糖、水苏糖3种成分)的高效液相色谱含量测定方法。方法:色谱柱Agilentzorbax NH2(4.6mm×250mm,5μm),柱温25℃;流动相水-乙腈(30∶70),流速1.2mL·min-1;示差折光检测器,内部温度35℃。结果:蔗糖、棉子糖、水苏糖的线性范围分别为2.38～23.8μg,2.04～20.4μg,2.24～22.4μg;加样回收率(n=5)分别为96.4%,97.5%,97.4%。结论:本方法简便、灵敏、准确,可用于含大豆低聚糖保健食品中以上3种成分的含量测定和产品质量控制。
Objective：To develop an RP-HPLC-RID method for determination of soybean oligosaccharide（including sucrose,raffinose,stachyose）in functional foods.Methods：An Agilent zorbax NH2 column （4.6 mm×250 mm,5 μm）was applied; a mobile phase composing of acetonitrile and water（70∶30）was used,and the flow rate was 1.2 mL·min^-1; A RID detector was applied,and the inner temperature was set at 35 ℃.Results：The results were obtained that quantitative line range of three oligosaccharide was 2.38-23.8 μg,2.04-20.4 μg,and 2.24-22.4 μg respectively,and the average recovery （n=5） was 96.38%,97.52%, and 97.40% respectively.Conclusion：The method is specific,sensitive,simple and reproducible ,and can be used for the determination of the three ingredients in functional foods that claimed containing soybean oligosaccharides.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第11期2081-2085,共5页
Chinese Journal of Pharmaceutical Analysis
