摘要
以红花月见草茎段为外植体,探讨其最佳灭菌条件及丛芽增殖条件。结果表明,最适的灭菌条件为0.1%HgCl2灭菌7 min;最适的增殖培养基为MS+1.5 mg·L-1 6-BA+0.15 mg·L-1 NAA。
In order to develop the most suitable sterilization measures and reproduction conditions of Limpia evening primrose stems with axillary bud were cultured in vitro. Results showed that： explants which were immersed in 0.1% mercuric chloride（HgCl2）for 7 min could obtain low infection and damage rate. And MS＋1.5 mg·L-1 6-BA＋0.15 mg· L-1 NAA was the suitable proliferation medium.
出处
《南方园艺》
2010年第6期3-5,共3页
Southern Horticulture
基金
乐山师范学院校级青年教师科研启动项目Z07039
关键词
红花月见草
灭菌条件
丛芽诱导
Limpia evening primrose
sterilization measures
reproduction
