摘要
目的观察完全饥饿大鼠肠神经和肠传输功能改变,探讨饥饿对肠动力功能影响的肠神经机制。方法 50只雄性SD大鼠随机分为饥饿组(n=40)和对照组(n=10)。饥饿组按照饥饿时间(饥饿后3、57、、9d)分为4个亚组(n=10)。采用葡聚糖蓝染色法测定不同时相肠道色素的推进比率;采用免疫组织化学染色法测定各组相同部位回肠肌间神经丛内乙酰胆碱酯酶(AchE)、一氧化氮合酶(NOS)阳性神经元的分布密度。结果饥饿组各时间点肠道传输比率均明显低于对照组(P<0.01),且随饥饿时间延长呈进行性下降,于9d达最低点。饥饿组各时间点AchE阳性神经元分布密度明显低于对照组(P<0.01),并随时间延长进行性下降,于9d达最低点。饥饿组各时间点NOS阳性神经元分布密度与对照组相比明显升高(P<0.01),并随时间延长进行性升高,于9d达高峰。结论完全饥饿状态下,大鼠肠肌间神经丛胆碱能神经元及氮能神经元受损,肠道动力功能障碍,且与肠神经损伤变化基本一致。
Objective To observe the changes of enteric nervous system and intestinal transit in starved rats, and explore the neuromechanism of starvation on gastrointestinal motility. Methods Fifty male SD rats were randomly divided into starvation group （n= 40） and normal control group （n= 10）. Rats in every starvation subgroup （n= 10） were killed on the 3rd, 5th, 7th and 9th day after starvation, respectively. The rate of intestinal transit was assessed by dextran blue-2000 as an indicator. The spreading specimens of intestinal myenteric plexus of ileums were collected and histochemically stained by AchE and nicotinamide-adenine dinucleotide phosphate-D （NADPH-d） to observe the density distribution and AchE and nitric oxide synthase （NO,S） positive neurons staining. Data were analyzed by Statistical Analysis Software SPSS13. 0. Resets The intestinal transit rate and the density distribution of Ache positive neurons in starvation group were significantly lower than those in normal control group （P〈0. 0l） at every time point after starvation, and reduced progressively with time passed and reached the lowest on the 9th day. While the density distribution of NOS positive neurons changed inversely. Conclusion AchE and NOS positive neurons and intestinal transit rate will be damaged after starvation, which is coincident with the changes of enteric nervous system after starvation,
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2010年第11期1367-1369,共3页
Medical Journal of Chinese People's Liberation Army
关键词
饥饿
肠神经系统
乙酰胆碱酯酶
一氧化氮合酶
hunger
enteric nervous system
acetyleholinesterase
nitric oxide synthase
gastrointestinal motility
