摘要
对中国近海和日本近海共19尾白姑鱼(Pennahia argentata)线粒体细胞色素b(Cytochrome b,Cytb)基因全序列进行扩增与测定,白姑鱼个体的Cytb基因总长度均为1141bp。序列分析结果显示:Cytb基因全序列中共检测到40处核苷酸替代,全部为同义替换,且主要来自密码子第三位点。19尾白姑鱼个体共定义了14种单倍型。核苷酸组成分析表明:白姑鱼Cytb全序列的鸟嘌呤(G)含量较低,在第三密码子位点上表现得尤为明显。基于邻接法、最大简约法、最大似然法和贝叶斯法构建的系统树结果基本一致,中国近海和日本近海的白姑鱼明显分为2个单系群,两组群间的净遗传距离为0.019,基于Cytb2%/百万年分子钟计算,其分化时间约为95万年,中、日近海白姑鱼分化事件发生于晚更新世(Late Pleistocene)。本研究旨在探讨中、日近海白姑鱼组群的分类地位的遗传学依据,为白姑鱼渔业资源的管理与保护提供基础参考。
The mitochondrial cytochrome b（Cyt b） gene of Pennahia argentata from different locations was amplified by PCR and the sequence with total length of 1411 bp was sequenced and analyzed.According to the analysis,two distinct clades were detected,one in China＇s offshore waters and another in the Japan＇s offshore waters.Forty nucleotide substitutions were checked in the total sequences analyzed,and most of them were synonymous transitions at the third codon positions.Nucleotide composition analysis indicated a strong bias against guanine（G） in the complete cytochrome b gene,especially in the third codon positions.The net average genetic distance between Chinese and Japanese clades were 1.9% in this study.Applying cytochrome b divergence rate of 2%/MY,the divergence of the two clades occurred about 950 000 years before present（BP） in Late Pleistocene.The topology of Bayesian tree is similar to those of neighbor Joining tree,maximum parsimonious tree,and maximum likelihood tree,which indicated significant genetic divergency existed in white croaker between China＇s offshore and Japan＇s offshore.
出处
《中国水产科学》
CAS
CSCD
北大核心
2010年第6期1166-1172,共7页
Journal of Fishery Sciences of China
基金
国家自然科学基金项目(30471329)
关键词
白姑鱼
细胞色素B基因
遗传距离
分歧时间
中国近海
日本近海
Pennahia argentata
cytochrome b gene
genetic distances
divergence time
Japan＇s offshore
China＇s off shore
