摘要
热休克蛋白90(heat shock protein90,HSP90)是细胞内主要的伴侣蛋白,在激素信号转导、细胞分化、细胞增殖与凋亡、形态发生与进化、变态发育及应激防御等多重调节路径中发挥关键作用。采用实时荧光定量RT-PCR技术检测了牙鲆两种HSP90基因在仔鱼发育和在成年组织中的表达变化。结果表明,HSP90α在成鱼骨骼肌、肠和胃中有较高的表达,而HSP90β在成鱼脑、脾脏和肾脏中有较高的表达。HSP90αmRNA水平在仔鱼变态期间迅速增加,至变态高峰G期达到最高水平;相反,HSP90β转录在仔鱼整个变态期间变化不是特别明显。鉴于甲状腺激素(thyroid hormone,TH)在牙鲆变态中的重要作用,还运用外源的TH及硫脲(thiourea,TU)处理牙鲆仔鱼来确定TH对HSP90基因转录的调节。与未处理组相比,HSP90α转录在TH处理8d和13d的仔鱼中显著增加,而在TU处理仔鱼中明显减少;但HSP90βmRNA水平不受药物处理影响。从上述结果分析,牙鲆中HSP90α的转录很可能被甲状腺激素上调,而且其在牙鲆的变态发育中发挥了重要作用。
HSP90 proteins are major chaperones that play pivotal roles in controlling multiple regulatory pathways such as hormone signaling,cell differentiation,cell proliferation and apoptosis,morphogenesis and morphological evolution,metamorphosis and stress defense.Using real-time fluorescent quantitative RT-PCR approach,expression profiles of HSP90 genes were examined during larval development and in adult tissues in Japanese flounder,Paralichthys olivaceus.In adult tissues,HSP90α was mainly expressed with higher transcript levels in skeletal muscle,intestine and stomach;but HSP90β exhibited higher mRNA levels in brain,spleen and kidney.During metamorphosis,HSP90α transcript increased distinctly with the highest mRNA levels at the metamorphosis climax;whereas HSP90β mRNA levels did not change.Due to the important role of thyroid hormone（TH）for the flounder metamorphosis,the transcriptional regulation of HSP90 genes by TH was evaluated using exogenous TH and thiourea（TU）treatment.Compared with untreated control,HSP90α transcript increased significantly in larvae sampled 8 and 13 days after TH treatment,and TU-treated larvae exhibited lower HSP90α mRNA levels.In contrast,the transcript levels of HSP90β did not vary after TH and TU treatment.Overall,these results demonstrate that HSP90α transcription is up-regulated by thyroid hormone and plays an important role during metamorphosis in Japanese flounder.
出处
《水产学报》
CAS
CSCD
北大核心
2010年第10期1478-1485,共8页
Journal of Fisheries of China
基金
国家自然科学基金项目(30571420)
上海市重点学科水生生物学建设项目(S30701)
上海海洋大学校青年基金项目(05-151)
