摘要
本实验通过高效液相色谱法(HPLC)分析植酸酶发酵液相对分子质量大于2000的物质中,植酸酶的含量为77.52%,植酸酶的相对分子质量约40000,实验分别选用截留相对分子质量为5000和100000的超滤膜处理植酸酶发酵液,超滤后植酸酶蛋白含量达90%。在25℃避光保存6个月,液态植酸酶样品浊度有所上升,但未见明显沉淀现象。
The high performance liquid chromatography（HPLC） was applied to test the fermentation liquid phytase.The results showed that ：the content of phytase was 77.52 % among all the components,which molecular weight were over 2000.The molecular weight of phytase was about 40000.The phytase fermentation liquid was treated by ultrafiltration membrane with molecular weight cut off（MWCO） at 5000 and 100000,respectively,the content of phytase after ultrafiltration could be over 90 %.The turbidity of liquid phytase was increased when stored in 25 ℃ over 6 months,but there was no precipitation in the stored samples.
出处
《中国饲料》
北大核心
2010年第22期27-29,共3页
China Feed
基金
温州市科技项目(G20080133)
关键词
液态植酸酶
稳定性
膜处理
liquid phytase
stability
membrane treatment
