摘要
为优化培养条件,研究含氮(BG11)和无氮(BG110)培养基对发菜(Nostoc flagelliforme)的色素、藻胆蛋白及胞内多糖和胞外多糖(EPS)含量的影响。结果显示:含氮组发菜的叶绿素a(Chl a)、类胡萝卜素(Carotenoids)、藻蓝蛋白(PC)、别藻蓝蛋白(APC)、藻红蛋白(PE)的含量均显著高于无氮组;胞内及胞外多糖含量无氮组高于含氮组,两者胞内多糖含量最大分别为1.03mg/mL和0.78mg/mL,EPS含量最大分别为243.8μg/mL和74.9μg/mL。表明氮元素的缺乏对发菜的色素合成有抑制作用,但在一定程度上能够促进胞内及胞外多糖的合成和分泌。
Cultivation of Nostoc flagelliforme in nitrogen-containing （BG11） and non-nitrogen-containing （BG110） media was conducted for figuring out the effects of nitrogen on the growth and the contents of phycobiliprotein （PC）,allophycocyanin （APC）,phycoerythrin （PE） and endo-and exopolysaccharides of Nostoc flagelliforme were investigated. The results showed that after 20 d cultivation,compared to BG110 medium,N. flagelliforme cultured in BG11 medium had much higher contents of Chl a,carotenoides,PE,APC and PC,but much lower contents of endo-and exo polysaccharides. The maximum content of endopolysaccharides was 1.03 mg/mL in BG110 and 0.78 mg/mL in BG11,while that of exopolysaccharides was 243.8μg/mL in BG110 and 74.9 μ g/mL in BG11. These results indicate that nitrogen deficiency may inhibit the growth of N.flagelliforme,but promote the synthesis of endo-and exopolysaccharides to a certain extent.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第19期92-95,共4页
Food Science
基金
国家自然科学基金项目(30671611)
苏州市科技局应用藻类学重点实验室基金项目(SZS0806)
关键词
发菜
氮
多糖
藻胆蛋白
Nostoc flagelliforme
nitrogen
polysaccharide
phycobiliprotein
