摘要
目的：采用ＨＰＬＣ测定妇舒液中黄芩苷含量，以控制该制剂的质量。方法：用十八烷基键合硅胶柱分离黄芩苷，流动相为甲醇４ｇ·Ｌ－１磷酸（５０∶５０）；检测波长２７８ｎｍ。结果：黄芩苷峰与对硝基苯酚峰的分离度为４．５；理论塔板数以黄芩苷峰计算为３８００；线性范围２０～９００ｎｇ（ｒ＝０．９９９９）；平均回收率为９９．８８％，ＲＳＤ＝１．５４％（ｎ＝５）。结论：本法简便、快速，适用于该产品质量分析检验。
OBJECTIVE:To domminate the quality of Fushuye,the contents of baicalin in Fushuye was determined by HPLC.METHOD:Baicalin was separated on Spherisorb C 18 column and detected at 278 nm,by using methanol 4g·L -1 phosphoric acid (50∶50) as mobile phase.RESULTS:The resolution was 4.5.The number of theoretical plates calculaled for baicalin peak was 3800.The standard curve was linear over the range of 20 ng～900ng,with the correlation coefficient 0.999 9.The average recovery was 99.88% with RSD =1.54%( n =5). CONCLUSION:The method is simple and fast,and can be used for quantitative analysis of Fushuye.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1999年第4期265-266,共2页
Chinese Pharmaceutical Journal
