摘要
以大花序桉优株无菌种子的下胚轴为外植体,研究其组培快繁技术。结果表明最适合大花序桉的基本培养基为ER培养基,最适的增殖培养基为ER+6-BA 0.5 mg/L+ZT 0.2 mg/L+NAA 0.3 mg/L,增殖系数达到3.8,不定芽生长整齐、健壮;最适的生根培养基为1/2ER+ABT12.5 mg/L+IBA 0.5 mg/L,生根率86%以上,每株平均能长4条根,平均根长2.2 cm,根系健壮。最适宜大花序桉移栽的基质是红心土,移栽成活率可达93%以上。
The Hypocotyls were used as explants to study the tissue culture and rapid propagation of Eucalyptus cloeziana in the experiment.The results were as follows： the best basic medium for Eucalyptus cloeziana was ER;the optimal subculture medium was ER＋6-BA 0.5 mg/L＋ZT 0.2 mg/L＋NAA 0.3 mg/L,in which the propagation coefficient reached 3.8,and the adventitious buds grew evenly and strongly.The optimal rooting medium for test-tube plantlets was 1/2ER＋ABT1 2.5 mg/L＋IBA 0.5 mg/L,and the rooting rate reached 86% in it.Per plantlet could produce 4 strong roots which average length was 2.2 cm.The optimum medium for Eucalyptus cloeziana plantlet transplantation was laterite,and the transplanting survival rate of the plantlets reached 93% in it.
关键词
大花序桉
下胚轴
组培快繁
Eucalyptus cloeziana
hypocotyl
tissue culture and rapid propagation
