摘要
从土壤中筛选到一株高效絮凝剂产生菌,经生理生化实验鉴定及16S rDNA序列分析,X14被鉴定为地衣芽胞杆菌（Bacillus licheniformis）。经培养条件优化,其最佳的培养条件为：淀粉10 g/L,氯化铵0.51 g/L,3 g/L K2HPO4和1 g/L KHPO,初始pH值为8,接种量采用1%,摇床转速采用140~160 r/min的变速控制,37℃培养48 h。
A bioflocculant-producing bacterium X14 was screened,which was identified as Bacillus licheniformis by 16S rDNA sequencing and its biochemical and physiological characteristics.The optimal culture conditions for Bacillus licheniformis X14 production are starch 10 g/L,NH4Cl 0.51 g/L,3 g/L K2HPO4,1 g/L KH2PO4,initial pH 8,inoculum size 1%（V/V）,culture temperature 37℃,culture time 48 h and shaking speed adopted variable-speed control from 140 r/min to 160 r/min.
出处
《环境工程学报》
CAS
CSCD
北大核心
2010年第11期2515-2518,共4页
Chinese Journal of Environmental Engineering
基金
水利部公益性行业专项科研经费(200801065)
关键词
地衣芽孢杆菌
微生物絮凝剂
絮凝活性
培养条件优化
Bacillus licheniformis
bioflocculant
flocculating activity
culture condition optimization
