摘要
筛选出了一株适用于石化污水处理的异养硝化-好氧反硝化产微生物絮凝剂菌株HAD-2,鉴定其为门多萨假单胞菌(Pseudomonas mendocina),考察了其最佳硝化条件、反硝化性能及在模拟污水中的脱氮能力。菌株为耐热菌,偏碱性(pH=8.5)和高碳氮比(25∶1)时硝化性能最佳。在异养硝化体系中,12 h时菌株对氨氮的去除率达到92.29%,硝酸盐和亚硝酸盐积累少;在反硝化体系中,12 h时菌株对亚硝酸盐和硝酸盐的去除率分别达到86.40%和84.92%;在模拟废水中,48 h时菌株对氨氮、硝态氮和亚硝态氮的降解率分别达到95.25%、65.47%和72.40%。菌株在多种培养基中可产微生物絮凝剂,在葡萄糖培养基中絮凝能力最佳,絮凝率为94%。
The given paper is concerned with the identification and characterization of a nitrogen removal bacterial strain with the flocculate capability in the oil-refinery sewage treatment. For the said purpose,we have managed to isolate a heterotrophic nitrification and aerobic denitrification bacteria strain HAD-2 with the flocculating capability from the active sludge of a petrochemical sewage treatment plant. What is more,we have also managed to amplify and sequence the 16 S rRNA gene and housekeeping gene rpo B. Strain HAD-2 is identified as Pseudomonas mendocina by the phylogenetic tree and physiochemical analysis. We have also done such processing treatments of the nap A gene,which is the function gene of the aerobic denitrification. The results of our study have confirmed the taxonomic status and aerobic denitrification ability of the strain HAD-2,with the best nitrification conditions,the denitrification capability and the nitrogen removal rate in the simulated sewage being carefully analyzed. The strain HAD-2 is by nature a kind of thermoduric bacteria which grow under the temperature of 45 ℃. Its ammonium removal rate can be promoted to the highest extent under alkaline（ at the p H value of 8. 5） and high C/N ratio（ 25： 1） conditions. In the heterotrophic system,the ammonia nitrogen removal rate of strain HAD-2 can be raised up to 92. 29% in a period of 12 h with little nitrite and nitrate accumulation. On the other hand,in the aerobic denitrification system,the nitrite and nitrate nitrogen removal rate can be upgraded to 86. 40% and 84. 92% as against their original. Furthermore,in the stimulated sewage system,the strain can also increase its removing rates to 95. 25% of ammonium,65.47% of nitrate and 72. 40% of nitrite nitrogen in a period of 48 h. Furthermore,strain HAD-2 can be made to produce the flocculation in various media,with the flocculate rate being up to92% in LB media and 88% in the stimulated sewage,while the highest flocculate rate can be upgraded to 94% in the glucose media. Strain
出处
《安全与环境学报》
CAS
CSCD
北大核心
2017年第3期1117-1124,共8页
Journal of Safety and Environment
基金
国家自然科学基金项目(30901108)
山东省自然科学基金项目(ZR2009EQ007)
中央高校基本科研业务费专项(15CX02014A)
关键词
环境工程学
异养硝化好氧反硝化
门多萨假单胞菌
产微生物絮凝剂
石化污水
environmental engineering
heterotrophic nitrification and aerobic denitrification
Pseudomonas mendoncina
flocculants production
petroleum sewage
