摘要
目的探讨表皮生长因子受体1(ErbB1)与ErbB3基因在新疆经典型Kaposi肉瘤(kaposi′s sarcoma,KS)发病过程中的表达情况。方法分别采用实时荧光定量PCR和免疫组化技术,对17例新疆经典型KS患者的肿瘤组织和瘤旁正常组织中ErbB1与ErbB3基因的表达进行检测。结果 ErbB1,ErbB3基因在KS肿瘤组织中mRNA的表达均低于瘤旁正常组织中的表达,差异有统计学意义(P<0.05);在KS与KS瘤旁正常组织中,ErbB1蛋白阳性表达率分别为11.8%和94.1%;ErbB3蛋白阳性表达率分别为17.6%和94.1%,ErbB1,ErbB3蛋白在KS中的表达明显低于在KS瘤旁正常组织中的表达,差异均有统计学意义(P<0.005)。结论 ErbB1和ErbB3基因在KS组织中呈现低度表达或不表达,提示其在新疆经典型KS肿瘤细胞增殖中可能作用有限。
Objective To investigate the possible mechanism of ErbBl and ErbB3 genes in the progress of classic Kaposi＇s sarcoma（KS） in Xinjiang. Methods Real-time PCR and immunohisto chemical technique were used to detec! ErbB1 and ErbB3 expression in 17 cases of KS and 17 cases of KS adjacent normal tissues. Results ErbBl and ErbB3 mRNA expression in tumor tissues of KS patients were lower than that in normal skin tissues, the difference was possessed of statistical significance（ P 〈0. 05 ）. The positive expression rates of ErbB1 in KS and KS adjacent normal tissues were 11.8% and 94. 1% ;the positive expression rates of ErbB3 in KS and KS adjacent normal tissues were 17.6% and 94.1%. The expressions of ErbB1 and ErbB3 proteins in KS were lower than those in KS adjacent normal tissues, there was statistical differences （P 〈 0. 005）between these two groups. Conclusion ErbB1 and ErbB3 were slightly or negative expressed in KS tissues, it indicates that they have slight function probably in the cell proliferation of classic KS in Xinjiang.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2010年第11期1007-1010,共4页
The Chinese Journal of Dermatovenereology
基金
国际合作项目(2005DFA30780)
石河子大学高层次人才科研启动资金专项(RCZX200787)
