摘要
目的:研究丹参对体外培养的人牙周膜成纤维细胞骨保护素(OPG)表达的影响。方法:原代培养人牙周膜成纤维细胞,传代至第5代,采用反转录聚合酶链反应(RT-PCR)及酶联免疫吸附试验(ELISA),分别检测不同浓度丹参对体外培养的人牙周膜成纤维细胞OPGmRNA表达及上清中OPG蛋白含量改变的影响,采用SPSS15.0软件包对数据进行统计学分析。结果:不同浓度丹参作用1h后,丹参处理组人牙周膜成纤维细胞OPGmRNA表达均较对照组增强(P<0.05);不同浓度丹参作用12h后,丹参处理组上清液中OPG蛋白质含量显著高于对照组(P<0.05)。结论:丹参能够促进体外培养的人牙周膜成纤维细胞合成并分泌OPG。
PURPOSE：To study the effect of Salvia Miltiorrhiza Bunge on the expression of osteoprotegerin （OPG） in cultured human periodontal ligament fibroblasts.METHODS：Primary culture of human periodontal ligament fibroblasts was established and the fifth passage cells were used in this study.Concentration-dependent effect of Salvia Miltiorrhiza Bunge on OPG mRNA and OPG protein secretion were determined by reverse transcription-polymerase chain reaction （RT-PCR） and enzyme linked immunosorbant assay （ELISA）, respectively.The data were analyzed with SPSS15.0 software package.RESULTS：OPG mRNA expression was increased after 1h exposure to various concentrations of Salvia Miltiorrhiza Bunge compared with the control group （P〈0.05）.OPG protein secretion was significantly increased after 12h exposure to various concentrations of Salvia Miltiorrhiza Bunge compared with the control group （P〈0.05）.CONCLUSION：Salvia Miltiorrhiza Bunge can up-regulate the expression of OPG in cultured human periodontal ligament fibroblasts.
出处
《上海口腔医学》
CAS
CSCD
2010年第5期530-533,共4页
Shanghai Journal of Stomatology
关键词
牙周膜成纤维细胞
丹参
骨保护素
Periodontal ligament fibroblast； Salvia Miltiorrhiza Bunge； Osteoprotegerin
