摘要
重氮法合成苏丹红Ⅰ的结构类似物,通过活性酯法将苏丹红Ⅰ的结构类似物偶联到载体蛋白上制备人工抗原,用所制备的人工抗原免疫BALB/c小鼠,采用细胞融合的方法制备苏丹红Ⅰ单克隆抗体,结果成功筛选到一株抗苏丹红Ⅰ单克隆抗体,经鉴定为IgG2a型,并建立间接竞争ELISA检测方法,线性范围为0.5～10ng/mL,检测下限为0.1ng/mL,加标回收率为52.3%～110%,变异系数为3.2%～8.9%。
A SudanⅠanalog was synthesized by diazotization and conjugated to bovine serum albumin（BAS） or keyhole limpet hemocyanin（KLH） by active ester method and a BAS based detection antigen and a KLH based immune antigen were obtained.A competitive enzyme-linked immunosorbent assay（ELISA） was established to detect the immunogenity of the immune antigen.BALB/c mice were immunized with the immune antigen for antibody generation.For the preparation of anti-Sudan Ⅰ monoclonal antibodies,cell fusion was used.An indirect competitive ELISA assay was developed.A linear relationship was observed over the concentration range of 0.5 to 10 ng/mL and the detection limit was 0.1 ng/mL.The average recovery rates of Sudan Ⅰ in red hot chili pepper powder spiked at 6 different levels were between 52.3% and 110% with variation coefficients varying from 3.2% to 8.9%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第18期258-261,共4页
Food Science
基金
江西省科技厅科技项目(GJJ10595)
江西科技师范学院博士科研启动基金项目
