摘要
目的:优化倒卵叶五加多糖提取分离工艺。方法:水提醇沉提取多糖,正交实验设计,探讨影响多糖提取分离工艺的诸因素。结果:倒卵叶五加多糖最佳提取分离工艺为用10倍水提取3次,每次1h。醇沉分离工艺最佳条件为乙醇浓度85%,料液比1∶2(g/mL),静置14h。结论:倒卵叶五加多糖提取分离工艺稳定可靠,最优条件下多糖得率为466mg/100g生药。
Objective: To optimize the extraction and isolation technology of polysaccharides from Acanthopanax obovatus Hoo. Methods: Qrthogonal experiments were adapted to design and analyze. Results. The optimal extraction condition was 10 times water,extracted 3 times, lh for each time.The optimal isolation condition was 1:2 (g/mL) of solid-liquid ratio,85% ethanol, depositing for 14h.Conclusion.The extraCtion and isolation technology of polysaccharides was simple, stable and efficient. The yield of polysaccharides under optimal condition was 466mg/100g drug.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第11期298-300,共3页
Science and Technology of Food Industry
关键词
倒卵叶五加
正交实验
提取分离
多糖
Acanthopanax obovatus Hoo
orthogonal experiments
extraction and isolation
polysaccharide