摘要
目的设计能检测麻疹病毒、犬瘟热病毒、牛瘟病毒、海豹瘟热病毒、海豚瘟热病毒和小反刍兽疫病毒的基因芯片探针,制备芯片用于以上病毒的种特异性检测。方法在ncbi网站下载上述病毒全部基因组序列信息,利用生物学软件CLUSTAL W对从GenBank中获得的6种病毒所有序列进行比对,获得每种病毒有意义的特异序列,以此作为设计oligo探针的备选序列,利用生物软件Primer Premier 5.0设计探针。选择最优化探针,制备基因芯片,并进行特异性验证。结果获得6条长度均一,退火温度相近,特异性良好的种特异性寡核苷酸探针。结论利用GenBank数据库和生物学软件Primer Premier 5.0设计病毒检测基因芯片探针,是一种快速、有效的方法。
Objective To design and prepare the microarray probes for species specificities of measles virus, canine distem-per virus, rinderpest virus, seal distemper virus, dolphin distemper virus and Peste des petits ruminants virus. Methods All the sequences of genomes of above-mentioned viruses in GenBank were downloaded from ncbi web site and blasted by using biological software CLUSTAL W. The meaningful specific sequences of each virus were obtained and used as the candidate sequence for design of oligo probes by biological software Primer Premier 5. 0. Gene chip was prepared with the optimal probes and verified for specificity. Results Six oligo probes for species specificity, at even lengths with good specificities, were obtained, of which the temperatures for annealing were similar. Conclusion Design of microarray probes for viruses by using GenBank and biological software Primer Premier 5. 0 was rapid and effective.
出处
《中国生物制品学杂志》
CAS
CSCD
2010年第10期1132-1134,共3页
Chinese Journal of Biologicals
基金
军队医药卫生科研基金06G138