摘要
基因芯片技术具有高通量、高度平行性、高度自动化的特点。在对传染病病原体的研究中,基因芯片技术已应用于耐药性相关遗传多态性分析、基因分型、生物种系的遗传进化分析、宿主与病原体相互关系分析、病原体检测等。但在病原体检测方面,与检测细菌相比,基因芯片技术对病毒的高通量检测难度较大。简要介绍了目前基因芯片技术在病毒性病原体检测中的研究进展、所采用探针的类型及设计原则、基因芯片杂交结果的影响因素等。
DNA microarray technique is characterized by its high throughput,highly parallel and highly automatic.On research of infectious pathogens,it has been widely used on drug resistance related genetic polymorphism analysis,genotyping,phylogenetic analysis,interactions between the pathogens and their hosts,and detection of pathogens.It is more difficult to detect viral pathogens than to detect bacteria by DNA microarray.The research progress of DNA microarray on detection of viral pathogens,types of the probes and the influential factors on hybridization results were introduced here.
出处
《生物技术通讯》
CAS
2008年第4期600-603,共4页
Letters in Biotechnology
关键词
基因芯片
病毒
检测
鉴定
DNA microarray
virus
detection
identification
