摘要
目的川北地区大肠埃希菌的整合子携带情况及其与耐药性之间的关系。方法采用琼脂稀释法测定临床分离的180株大肠埃希菌对15种抗菌药物的敏感性，敏感、中介、耐药采用美国临床实验室标准化协会（CSLI）2006年公布的标准判定。PCR方法检测临床大肠埃希菌中的第一类整合酶基因（伽们，应用统计学方法比较第一类整合酶基因（加们阳性与阴性菌株的耐药性差异，分析I类整合子与耐药性形成的的相关性。结果180株大肠埃希菌对氨苄西林、环丙沙星、左旋氧氟沙星、庆大霉素、复方磺胺甲嗯唑、头孢唑啉、妥布霉素、氨苄西林／舒巴坦、头孢曲松的耐药率高，达32．9％～92.3％。临床分离180株大肠埃希菌中intI携带率为64．4％，intI阳性菌株对β-内酰胺类、喹诺酮类和氨基糖苷类等抗菌药物呈现多重耐药性。结论川北地区大肠埃希菌对临床常用抗菌药物耐药突出。intI携带率高，并且与耐药性的形成关系密切。
Objective To investigate the distribution of class I integrons and interpret the association of class I integron with drug resistance in Escherechia coli clinical isolates. Methods Agar dilution method was performed to detect the susceptibility to 15 different antimicrobial agents against 180 strains of Escherechia coli and the results were assessed with CSLI. Class 1 integron genes （intl） were detected by polymerase chain reaction. Results The resistant rates of 180 strains ofEscherichia coli were 32. 9%-92. 3% to ampicillin, ciprofloxacin, levofloxacin, gentamycin, trimethoprim-sulfamethoxazole, cefazolin, tobramicin, ampicillin/sulbactam, ceftriaxone. The incidence of class 1 integron （intl） was 64.4%. The intI-positive strains showed multidrug resistance to β-lactamases, quinolones and aminoglycosides. Conclusion The resistance of Escherechia coli were prominent for clinical antimicrobial in North of Sichuan. Class 1 integron was extensively found in Escherechia coli clinical isolates, and integrons played an important role in drug resistance.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2010年第10期784-787,共4页
Chinese Journal of Antibiotics
基金
四川省重点科技项目(No.2006J13及07ZA034)
