摘要
目的 建立高效液相色谱-柱后衍生法测定硫酸妥布霉素含量的方法。方法色谱柱为watersSunFireC18（4．6mm×150mm，5μm）；流动相为含戊烷磺酸钠和冰醋酸的硫酸钠溶液；柱温30℃，流速1．2mL／min。柱后衍生化试液为邻苯二甲醛溶液，流速0．5mL／min，衍生化反应温度60℃，荧光激发波长360nm，发射波长440nm。结果硫酸妥布霉素在4．15-62．28mg／L浓度范围内线性关系良好（r=0．9999），平均回收率0=9）为99．5％。结论该方法简便快速，结果准确可靠，重复性好，可用于硫酸妥布霉素的定量分析。
Objective To establish a method using HPLC with post-column derivatization for detecting tobramycin sulfate drops. Methods A Waters SunFire C18 column（4. 6mm×150mm, 5μm） was used, and the mobile phase was sodium sulfate solution （contain pentanesulfonate sodium salt and glacial acetic acid）, column temperature was 30℃, at a flow rate of 1. 2mL/min. OPA solution was used for post-column derivatization online, at a flow rate of 0. 5mL/min. The temperature of post-column reaction was set at 60℃, detected by fluorescence detector （λex 360nm, ）λem 440nm）. Results The calibration curve was linear in the range of 4. 15-62. 28mg/L, r-=0. 9999. The average recovery（n=9）was 99. 5%. Conclusion The method presented in this study showed good accuracy, more precision, simplicity and reproducibility as a recommendable option for quality control oftobramvcin sulfate drops.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2010年第10期771-773,共3页
Chinese Journal of Antibiotics
