摘要
背景:放射性肝损伤极大限制了放射治疗的临床应用。研究发现,肝损伤环境是骨髓间充质干细胞适宜的分化环境,提示通过骨髓间充质干细胞移植治疗放射性肝损伤成为可能。目的:验证骨髓间充质干细胞移植对大鼠急性放射性肝损伤中α-平滑肌肌动蛋白表达水平的影响。方法:分离培养雄性大鼠的骨髓间充质干细胞,取P3代细胞作为移植细胞。建立雌性SD大鼠急性放射性肝损伤模型。随机分成两组,在放疗结束后4h内,干预组经尾静脉输注骨髓间充质干细胞悬液,对照组输注等量生理盐水。采用免疫组织化学技术检测肝脏组织中的α-平滑肌肌动蛋白的阳性表达水平;采用原位杂交技术检测肝脏组织中性别决定基因Y(SrY)的存在;光镜观察肝脏组织的病理形态学变化。结果与结论:干预组大鼠肝右叶中α-SMA的平均阳性表达率低于对照组(P<0.05)。干预组大鼠肝右叶检测到携带SrY的阳性细胞多于肝脏左叶(P<0.05),对照组中未检测到携带SrY的阳性细胞。干预组大鼠肝右叶的损伤程度比对照组轻。结果证实,急性放射性肝损伤可以诱导雄性大鼠的骨髓间充质干细胞的"归巢",骨髓间充质干细胞对大鼠急性放射性肝损伤中ɑ-平滑肌肌动蛋白表达水平有下调作用。
BACKGROUND：Radiation-induced liver injury has greatly limited the clinical application of radiotherapy.Research demonstrated that liver injury environment is suitable for the differentiation of bone marrow mesenchymal stem cells （BMSCs）, which suggested that it is possible to treat radiation-induced liver injury with BMSCs transplantation.OBJECTIVE：To test effect of BMSCs transplantation on ɑ-smooth muscle actin （SMA） expression level in acute radiation-induced liver injury rats.METHODS：BMSCs were isolated from male rats, and subcultured.The third passage of BMSCs was selected as transplanted cells.Female Sprague Dawley rat models of acute radiation-induced liver injury were established, and randomly assigned to two groups.Within 4 hours following radiotherapy, BMSCs suspension was infused through caudal vein in the intervention group.An equal volume of saline was infused into the control group.ɑ-SMA expression was detected by immunohistochemistry in the liver.Sex determining gene Y （SrY） was determined using in situ hybridization technique.Pathomorphological change in the liver was observed under an optical microscope.RESULTS AND CONCLUSION：The positive expression rate of ɑ-SMA in right lobe of liver of intervention group was lower than control group （P〈0.05）.The existence of SrY was more in the right lobe of liver than that of in the left lobe of liver in intervention group（P〈0.05）.In control group, no SrY gene can be found.The injury degree of right lobe liver in intervention group was lower than control group.Results have verified that acute radiation-induced liver injury can lead BMSCs＇ homing in male rats.BMSCs can down-regulate the expression level of ɑ-SMA induced by acute radiation in liver injury rats.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第36期6744-6750,共7页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
新疆医科大学第一附属医院干细胞专项基金(2009GXB02)~~
