摘要
目的为充分利用有限的优质动物资源,缩短世代间隔,提高胚胎工程技术在生物领域中的应用效率。方法利用未性成熟小鼠模型和性成熟小鼠模型,分别进行PMSG/hCG超数排卵处理与体外受精;统计排卵率、受精率和囊胚发育率;观察小鼠卵巢的组织形态学变化,了解日龄对小鼠卵母细胞体外受精效果的影响,同时分析遗传因素在小鼠卵母细胞体外受精中的作用。结果未性成熟小鼠超数排卵的可用卵母细胞数量显著多于性成熟小鼠,且其卵巢上有腔卵泡的数量也明显多于性成熟小鼠;未性成熟小鼠卵母细胞的体外受精率显著低于性成熟小鼠,但囊胚发育率差异不显著。通过对性成熟小鼠的可用卵母细胞数进一步分析发现,高排卵组(平均可用卵母细胞数≥40枚)小鼠卵母细胞的体外受精率和囊胚发育率都显著高于低排卵组(平均可用卵母细胞数<40枚);且同窝小鼠的卵巢卵泡发育情况与排卵数基本一致。结论未性成熟小鼠卵巢对超数排卵反应的敏感性强,性成熟小鼠卵巢的超数排卵敏感性与遗传因素有关,且排卵性状的高低与卵母细胞的质量密切相关。
Objective Immature and mature mouse models were used and the effect of age on mouse in vitro fertilization were identified in order to make full use of high quality animal resources, to shorten the intervals between generations and efficiently to improve the embryo engineering technology. Methods The ovulation rate, the in vitro fertilization rate and the blastocyst rate were measured after pregnant mare serum gonadotropin （PMSG）/ human chorionic gonadotrophin（ hCG ） superovulation of both the immature and mature mice as well as the ovarian morphological changes were observed. At the same time, the role of genetic factor in the mouse in vitro fertilization was evaluated. Results The ovulation rate in immature mice was significantly higher than that in mature mice, and antral follicles in immature mouse ovaries were more than in mature mouse ovaries. There was a significantly lower fertilization rate from immature mouse oocytes than from mature mouse oocytes, however, there was no remarkable difference on the blastocyst rate between these two groups. Further analysis of usable oocytes in the mature mouse group found that the high response group to superovulation （ usable oocytes ≥40） had significantly higher fertilization rate and blastocyst development rate than those of the low response group to superovulation （usable oocytes 〈 40）. In addition, the ovulation rate arid the follicle development among different individuals in the same litter were similar. Conclusion There is a high response to superovulation in the immature mice, while in the mature group, the ovarian response to superovulation may be genetic background-dependent and closely related to the oocyte quality.
出处
《解剖学报》
CAS
CSCD
北大核心
2010年第5期719-723,共5页
Acta Anatomica Sinica
基金
吉林医药学院科研资助基金(2008-01)
关键词
卵巢
卵母细胞
超数排卵
体外受精
小鼠
Ovary
Oocyte
Superovulation
In vitro Fertilization
Mouse
