摘要
目的探讨代谢综合征幼鼠肝脏中皮质醇代谢关键酶5α-还原酶1及11β-羟类固醇脱氢酶1的表达及其对皮质醇代谢功能的影响。方法 3周SD幼鼠随机分为普通饮食组(NC组)、高脂组(FC组)及高脂高盐组(FSC组)3组。测体质量、体长、腹围、血压,观测内脏脂肪重量、血脂、血皮质醇等,行口服葡萄糖耐量试验及胰岛素释放试验评价血糖及胰岛细胞功能。取新鲜肝脏组织,测定5α-还原酶1及11β-羟类固醇脱氢酶1mRNA含量及两酶mRNA含量比值。结果高脂高盐组大鼠腹围、血压、内脏脂肪、空腹血糖、胰岛素水平均比对照组显著增加,血脂紊乱加重并表现出显著的胰岛素抵抗。高脂和高脂高盐组幼鼠5α-还原酶1mRNA增加而11β-羟类固醇脱氢酶1mRNA含量减少,两酶mRNA含量比值显著增加,上述指标均与血皮质醇水平显著相关。结论代谢综合征模型组幼鼠肝脏组织高表达5α-还原酶1及低表达11β-羟类固醇脱氢酶1可加重胰岛素抵抗和血脂紊乱,糖皮质激素代谢及调控异常可能与代谢综合征发生相关。针对性调控5α-还原酶1及11β-羟类固醇脱氢酶1表达和活性可能是代谢综合征的一种病因治疗方法。
Objective To investigate the gene expression of hepatic 5αreductase type 1 （5α-R1） and 11β- hydroxysteroid dehydrogenase type 1 （ 11β-HSD1 ）, and the ratio of these two enzyme （5α-R1/11β-HSDI ） in young rats with metabolic syndrome. Methods Weaned, 3 week old SD young rats were divided into three groups routine diet （control）, high fat diet （FC）, and high fat and salt diet （FSC） respectively. The body weight, length, waist circumference, and blood pressure were measured. The amount of fat around visceral organs was evaluated. The serum lipids and cortisol were measured. Oral glucose toleranee test and insulin release test were performed. Hepatic 5α-R1 and 11β-HSDI mRNA were assessed in fresh liver samples, and the ratio of 5α-R1/11β-HSD1 mRNA was calculated. Results In the FSC group, the waist circumference, blood pressure, amount of fat around visceral organs, blood glucose, insulin were significantly increased compared with the control group. In the FSC group, the hepatic 5α-R1 mRNA was significantly increased, 11β-HSDI mRNA was significantly decreased, and the ratio of 5α-RI/11β-HSD1 mRNA was increased. Serum cortisol was significantly related to 5α-R1 mRNA, 11β-HSD1 mRNA, and the ratio of 5α-R1/11β-HSD1 mRNA. Conclusions High hepatic 5α-R1 expression and low hepatic 11β-HSD1 expression may increase insulin resistance and dyslipidemia. Glucocorticoids metabolic disorder might be relateed to the metabolic syndrome in children. It is suggest that regulation of 5α-R1 and 11β-HSD1 expression might be an effective treatment for children with metabolic syndrome.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2010年第10期971-975,共5页
Journal of Clinical Pediatrics
