摘要
本文采用玻璃化法对蛇莓离体茎尖超低温保存进行了初步探讨。研究了低温锻炼时间、预培养时间、预处理时间、玻璃化液处理时间和液氮保存时间对超低温保存后成活率的影响。经优化,蛇莓的最高成活率可达(42.00±2.74)%。
A procedure had been studied on cryopreservation of Duchesnea indica (Andr.) Focke shoot-tips in vitro by vitrification. The influence of the treatment periods of cold temperature acclimation,pre-culture,pretreatment,PVS2 and cryopreservation in liquid nitrogen on survival rate after cryopreservation was studied. The best survival rate reached to (42.00±2.74)%.
出处
《植物生理学通讯》
CSCD
北大核心
2010年第9期953-956,共4页
Plant Physiology Communications
基金
国家自然科学基金(30900973)
关键词
蛇莓
茎尖
超低温保存
玻璃化
成活率
Duchesnea indica
shoot-tip
cryopreservation
vitrification
survival rate