摘要
目的探讨彩色多普勒成像技术(CDFI)与高频超声结合eFlow成像技术在评价鸡FGFR1重组蛋白联合低剂量吉西他滨抗小鼠肿瘤血管生成疗效中的价值。方法 40只小鼠皮下接种2×106个肿瘤细胞(Meth A模型),7 d后随机分成以下4组(10只/组):cFR蛋白疫苗联合低剂量吉西他滨治疗组(C+G组)、cFR蛋白疫苗单独治疗组(cFR组)、低剂量吉西他滨单独治疗组(G组)、生理盐水非治疗对照组(NS组)。于分组后第4周使用CDFI及高频超声eFlow显像显示小鼠肿瘤内微血管的走行和分布,并计算瘤内的血管和肿瘤的像素比(即血管/肿瘤像素比)。结果①40只小鼠种植肿瘤后均形成皮下明显的肿瘤结节。②eFlow成像较CDFI更能显示肿瘤内分支细小血管,彩色血流溢出少,敏感性更高。③C+G组、cFR组、G组、NS组肿瘤内的血管/肿瘤像素比(%)呈升高趋势,C+G组、cFR组、G组分别与NS组相比较,差异具有统计学意义(均P<0.05)。结论高频超声结合eFlow显像技术能够显示肿瘤微小血管生成情况,为评价抗肿瘤血管生成疗效提供了一种实时且无创的、敏感性好的方法。
Objective To assess the value of the chicken FGFR1 recombinant protein combined with low-dose gemcitabine in anti-tumor angiogenesis in mice by color Doppler imaging and high-frequency ultrasound combined with eFlow imaging.Methods Forty mice were inoculated subcutaneously with 2×106 tumor cells to establish Meth A model,and 7 days later they were randomly divided into 4 groups：combined treatment group（the chicken FGFR1 recombinant protein combined with low-dose gemcitabine,C＋G group）,group of recombinant protein vaccine of chicken FGFR1 alone（cFR group）,group of gemcitabine alone（G group）,control group（NS group）.Four weeks later,color Doppler imaging and high-frequency ultrasound combined with eFlow imaging were used to show the running and distribution of microvessels in mice tumors,and the vessels/tumor pixel ratio was calculated.Results ①Obvious subcutaneous nodules were seen in 40 mice after injection of Meth A tumor cells;②eFlow imaging could show smaller branches of tumor blood vessels,and had a higher sensitivity than CDFI;③The vessels/tumor pixel ratio（%）from C＋G group,cFR group,G group to NS Group was increased in turn.The vessels/tumor pixel ratio（%）in C＋G group or cFR group was lower than in control group with the difference being statistically significant（P0.05）.Conclusion High-frequency ultrasound combined with eFlow imaging can show tumor microvessel course and the distribution of small branches of tumor blood vessels.It can provide a real-time and a new non-invasive method for evaluating the effect of anti-tumor angiogenesis.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2010年第5期684-687,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金(No.30660055
No.30900663)
教育部"新世纪优秀人才支持计划"基金(NCET-08-0657)
海南省自然科学基金(No.310043)资助项目
