摘要
目的了解Antigen43/成纤维细胞生长因子1型受体(Ag43/FGFR1)重组嵌合蛋白作为疫苗能否诱导小鼠产生抗自身FGFR1抗体。方法利用重组的Ag43/FGFR1嵌合蛋白作为疫苗免疫BALB/c小鼠,分别用Westernblot、酶联免疫吸附实验(ELISA)和酶联免疫斑点实验(ELISPOT)方法检测小鼠血清中产生的抗体及其亚型和脾脏中分泌抗FGFR1抗体的特异B淋巴细胞情况。结果Western blot和ELISA检测发现,Ag43/FGFR1蛋白免疫组可以有效诱导产生抗自身FGFR1抗体,而小鼠FGFR1自身蛋白对照组、Ag43蛋白对照组、生理盐水对照组均未发现抗自身FGFR1抗体。ELISPOT检测发现,与各对照组相比,Ag43/FGFR1蛋白免疫组分泌抗自身FGFR1抗体的B淋巴细胞数目明显增多(均P<0.01)。ELISA检测发现主要抗体亚型IgG1和IgG2b明显升高。结论Ag43/FGFR1重组嵌合蛋白质疫苗免疫小鼠能够诱导产生抗小鼠FGFR1的自身抗体,可以考虑应用于抗肿瘤血管生成研究。
Objective To explore the possibility that a recombinant Ag43/FGFR1 chimeric protein vaccine induces production of autoantibodies against self-FGFR1 in BALB/c mice.Methods The BALB/c mice were immunized with the recombinant Ag43/FGFR1 chimeric protein as a vaccine.Autoantibodies against self-FGFR1 were determined by enzyme-linked immunosorbent assay（ELISA）,Western blot analysis and enzyme-linked immunospot assay（ELISPOT） respectively.Results Autoantibodies against mouse FGFR1 were identified by Western blot analysis and ELISA.Compared with the three control groups,the number of APBCs,which were detected by ELISPOT assay,were significantly increased in the spleens of mice immunized with Ag43/FGFR1（P〈0.01）.IgG1 and IgG2b,which were detected by ELISA,were the major subclasses and substantially increased in response to Ag43/FGFR1 compared to control group.Conclusion The recombinant Ag43/FGFR1 chimeric protein used as a vaccine could induce autoantibodies against self-FGFR1 in mice,which provided a basis for the active immunotherapy of tumor angiogenesis.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2009年第4期438-440,445,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金(No.30660055
30460136)
教育部新世纪优秀人才基金(NCET-08-0657)
海南省自然科学基金(No.30864)
教育部科学技术研究重点项目(No.209097)资助项目
