摘要
在pH8．0的Tris—HCl介质中，三磷酸腺苷（ATP）能诱导十六烷基三甲基溴化铵（CTAB）包被的正电金纳米颗粒（AuNPs）聚集，引起其表面等离子体共振吸收及等离子体共振散射变化．通过扫描电子显微镜、动态光散射及Zeta电位表征了金纳米颗粒的聚集，讨论了ATP与金纳米颗粒的结合模式，确定了三磷酸腺苷与正电金纳米颗粒的配位作用．在此基础上建立了表面等离子体共振吸收定量检测ATP的方法．方法的线性范围为4．0-80μmol／L，检出限为0．82μmol／L．相同浓度的ADP，AMP，UTP，CTP和GTP不干扰测定．方法用于人体尿样ATP的检测，回收率在94.4％～123％之间，相对标准偏差RSD小于2．5％．与传统ATP检测方法相比，该方法简单快速，选择性好．
It was found adenosine triphosphate （ATP） could induce the aggregation of cetyltrimethylammonium bromide （CTAB） capped gold nanoparticles （AuNPs） in Tris-HCl buffer pH 8.0, which caused the changes of surface plasma resonance absorption and resonance light scattering. Scanning electron microscopy （SEM）, dynamic light scattering （DLS） and （-potential have been used to characterize the aggregation of AuNPs, and how ATP molecule combine with AuNPs has been discussed. With the changes of surface plasmon resonance absorption of AuNPs, a new method for the determination of ATP has been developed in linear range of 4.0-80 μmol/L, with the detection limit of 0.82 μmol/L. It was found that ADP, AMP, UTP, CTP, GTP had no interfering effects in the determination. The method could he applied for the determination of ATP in urine samples with the recovery between 94.4% and 123%, and R.S.D. less than 2.5%. Comparing with other methods, the proposed method reported here is simple, rapid, and highly selective.
出处
《科学通报》
EI
CAS
CSCD
北大核心
2010年第27期2683-2689,共7页
Chinese Science Bulletin
基金
国家自然科学基金重大研究计划资助项目(90813019)
关键词
金纳米颗粒
十六烷基三甲基溴化铵
三磷酸腺苷
等离子体共振吸收
gold nanoparticles, cetyitrimethylammonium bromide, adenosine triphosphate, plasmon resonance absorption
