期刊文献+

动物转基因技术的研究现状与应用 被引量:1

下载PDF
导出
摘要 转基因动物就是指通过人工的方法将外源基因导人动物染色体基因组,使之稳定表达并能遗传给后代的一类动物。转基因技术的一般方法有原核期胚胎的显微注射法(Microjection)、逆转录病毒载体法、精子载体法、体细胞核移植技术、胚胎干细胞介导的基因转移,这几种方法各有其公优缺点,在动物转基因上均有不同的应用,目前在动物抗病育种、建立诊断和治疗人类疾病的动物模型、利用转基因动物生产药用蛋白质等方面应用越来越广泛。
作者 杨曾
出处 《畜禽业》 2010年第10期36-37,共2页 Livestock and Poultry Industry
  • 相关文献

参考文献15

  • 1胡建宏,李青旺,王立强,江中良,于永生,解广勤.转基因动物研究进展[J].家畜生态,2004,25(2):50-53. 被引量:7
  • 2朱玉贤,李毅编著..现代分子生物学[M].北京:高等教育出版社,1997:481.
  • 3冯斌,谢先芝编著..基因工程技术[M].北京:化学工业出版社,2000:157.
  • 4Chan A W.S,Chong K Y ,Martinovich C,et al.Transgenic monkey produced by retroviral gene transfer into mature ooyetes.Science, 2001,291:309-312. 被引量:1
  • 5Haskell R E et al.Efficient production of transgenic cattle by retroviral infection of early embryos.Mol Reprod Dev,1995,40:386- 390. 被引量:1
  • 6Anthony W ,Homan E,Ballou J C,et al Transgenic cattle produced by reverse-transcribed gene transfer in oocyte.Proc Natl Acad Sci USA,1998, 95:14 028-14 033. 被引量:1
  • 7Brackett G,Baraunska W.Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization E J.Proc Natl Acad Sci.USA,1997.2: 253-257. 被引量:1
  • 8袁仕善,周智广.阳离子脂质体与基因转移[J].生理科学进展,1997,28(2):163-165. 被引量:12
  • 9Anthony G F P,et al.Mammalian transgenesis by intracytoplasmic sperm injection. Science,1999,284:1 180-1 183. 被引量:1
  • 10Schnieke A E,et al.Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts .Science,1997,28:2 130-2 133. 被引量:1

二级参考文献60

共引文献44

同被引文献26

  • 1GORDON J W,SCANGOS G A, PLOSKIN D J, et al. Genetic trans formation of mouse embryo by microinjeetion of purified DNA[J]. Proc Natl Acad Sci USA,1980,77(12):7380-7384. 被引量:1
  • 2DIAKUN G P, FAIRALL L, KLUG A. EXAFS study of the zinc-binding sites in the protein transcription factor m A[J]. Nature, 1986,324 (6098) : 689-699. 被引量:1
  • 3KIM Y G,CHA J, CHANDRASEGARAN S. Hybrid restriction enzymes: Zinc finger fusions to Fok I cleavage domain[J]. Proc Natl Acad Sci USA,1996,93(3):l156-1160. 被引量:1
  • 4URNOV F D, MILLER J C, LEE Y L, et al. Highly effi- cient endogenous human gene correction using designed zinc- finger nucleases[J]. Nature,2005,435(7042):646-651. 被引量:1
  • 5JANENISCH R, MINTZ B. Simian virus 40 DNA sequences in DNA of heathy adult mice derived from preimplantation blasto- cysts injected with viral DNA [J]. Proc Natl Acad Sci USA, 1974,71(4) : 1250-1254. 被引量:1
  • 6SALTER D W, SMITH E F, HUGHESS S H, et al. Trans- genie chickens: Insertion of retroviral genes into the chicken germline[J]. Virology, 1987,157 ( 1 ) :236-240. 被引量:1
  • 7BIERY K A, BONDIOLI K R, DEMAYO F J, et al. Gene transfer by pronuclear injection in the bovine[J]. Thercogenol- ogv, 1995,29 ( 1 ) : 224-229. 被引量:1
  • 8BRACKETY B G, BARAUNSKA W,SAWICK W,et al. Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization [J]. Proc Natl Acad Sci USA, 1971,68(2) :353-357. 被引量:1
  • 9LAVITRANO M, LAMAIONI A, FAZI V M,et al. Sperm ceils as vectors for introcuting foreign DNA into eggs: Gene transformation of mice[J]. Cell, 1989,57(5) :717-723. 被引量:1
  • 10SQUIRES E J,DRAKE D. Liposome-mediated and transfer to chicken sperm cells[J]. Ani Biotech,1993,3(4) :71-88. 被引量:1

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部