摘要
目的：血小板活化因子（ＰＡＦ）拮抗剂能改善局部脑缺血所致功能和组织损伤，本文在沙土鼠单侧脑缺血及大鼠大脑中动脉凝闭的局部脑缺血观察了缺血后大脑组织ＰＡＦ的变化。方法：ＰＡＦ的测定采用薄层层析进行ＰＡＦ分离提取，用生物法测定ＰＡＦ含量的变化。结果：１沙土鼠右侧颈总动脉结扎３ｈ再灌２ｈ，右侧大脑组织ＰＡＦ含量明显高于左侧。２大鼠左侧脑中动脉凝闭后４８ｈ，左侧大脑组织ＰＡＦ含量明显高于右侧。结论：ＰＡＦ在局部脑缺血继发神经元损伤中可能有一定作用。
AIM:Platelet-activating factor(PAF) bas been reported to be an active mediator of neuronal damage in regional cerebral ischemia on the basis of indirect pharmacological data from PAF antagonists. The direct measurement of PAF has not been reported previously in regional cerebral ischemia. It prompted us to investigate the change of PAF in unilateral ischemia in gerbils and regional ischemia in rats.METHODS:Thin layer chromatography was combined with biological methods for the determination of PAF in brain after cerebral ischemia.RESULTS:(1) The PAF content of ischemic brain was increaesd significantly in gerbils subjected to 3 h unilateral cerebral ischemia followed by 2h reperfusion respectively(4.40±0.90) and (1.30±0.40) pg/g, P <0.05. (2)The PAF content of ischemic brain was increased significantly also in rats subjected to 48 h regional ischemia respectively(5.74±0.75) and (0.76±0.05)pg/g, P <0.05. CONCLUSION:PAF could contribute to secondary neuronal damage after regional cerebral ischemia.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第5期392-394,共3页
Chinese Journal of Pathophysiology
基金
国家自然科学基金
关键词
血小板活化因子
脑缺血
大鼠
沙土鼠
Platelet activating factor
Cerebral ischemia
Gerbillinae
Rats
