摘要
利用体外培养技术，以Ｋ５６２细胞为实验模型，通过Ｋ５６２细胞的生长曲线、细胞形态、乳酸脱氢酶活性、血红蛋白测定等，观察了苦参提取液对Ｋ５６２细胞的诱导分化作用。以ＭＴＴ法结合细胞形态探讨苦参提取液作用Ｋ５６２细胞的有效分化浓度及有效作用时间，研究结果表明：１２ｍｇ／ｍｌ浓度的苦参提取液作用Ｋ５６２细胞二天，从形态到代谢都发生了变化。ＬＤＨ活性明显降低；血红蛋白生成；细胞增殖明显受到抑制。电镜形态提示，细胞向似红细胞系、粒细胞系及单核细胞系分化。
Using culture assay in Vitro, K562 cell strain as anexperimental model, We observed the effect
of sophoa extract on induced differentiation of K562 cell strain by cell growth curve, cell
morphology, Latic dehydrogenase activity assay and hemoglobin determination. The effective
concentration and time by MTT assay combined with cell morphology was studied. Results
showed that K562 cell bad been acted on by sophoa extract 12mg/ml for two days. Its
morphology and metabolism were changed. Their LDH activity lowered obviously; the Hb was
produced by these cells; the proliferation of the cell was suppressed. Electron microscopy
Showed: the cells differentiate toward the primitive cell with erythrocyte , granulocyte and
monocyte lineages
出处
《重庆医科大学学报》
CAS
CSCD
1999年第2期119-123,共5页
Journal of Chongqing Medical University
关键词
K562细胞
诱导分化
白血病
苦参提取液
Sophoa flavescans Ait: K562 cell strain
Induction of
differentiation
