摘要
目的探讨神经营养因子(neurotrophin,NT)-3在肺炎链球菌性脑膜炎脑组织及抗生素治疗后的表达变化特点。方法将3周龄SD大鼠46只(雌、雄不限,昆明医学院动物研究所提供),采用(0.15～0.30)mL/100 g 10%水合氯醛腹腔注射麻醉后,建立肺炎链球菌性脑膜炎脑损伤模型(n=34)。建模成功后,根据是否接受进一步治疗,随机分成感染组(n=20)、治疗组(n=14)。其余纳入正常对照组(n=12)。各组间大鼠的平均体重和鼠龄比较,差异无显著意义(P>0.05)。①46只大鼠均行脑池穿刺,感染组和治疗组接种50μL肺炎链球菌混悬液,正常对照组接种50μL生理盐水。观察神经营养因子-3在脑组织中的表达。经自然病程,感染组大鼠分别于接种后24 h和第5天处死(24 h感染组,n=8;5 d感染组,n=12)。②将治疗组分为抗生素治疗组(n=8)和抗生素对照组(n=6),前者给予头孢曲松皮下注射治疗3 d后,与正常对照组神经营养因子-3在脑组织中的表达进行比较。后者不做任何处理。③正常对照组于接种生理盐水后24 h(24 h正常对照组,n=6)和5 d(5 d正常对照组,n=6)分别处死。结果在脑皮质和海马中,24 h感染组,与正常对照组比较,神经营养因子-3明显下降(P<0.01),第5天回升(5 d感染组),但仍未达到正常对照组水平(P<0.05)。抗生素治疗组神经营养因子-3蛋白表达水平低于抗生素对照组,差异有显著意义(P<0.05),与5 d感染组比较,差异无显著意义(P>0.05);抗生素对照组与5 d正常对照组神经营养因子-3蛋白的表达比较,差异无显著意义(P>0.05)。结论大鼠罹患肺炎链球菌性脑膜炎时,神经营养因子-3在脑组织中的表达下降。在肺炎链球菌性脑膜炎急性脑损伤中,神经营养因子-3对神经的保护支持作用可能减弱。在肺炎链球菌脑膜炎中,抗生素治疗后,神经营养因子-3在脑组织中的表达仍处于较低水平,在肺炎链球菌性脑膜炎脑损伤中,神经营养因子-3的内�
Objective To investigate the expression of neurotrophin-3（NT-3） expression in brain injury after antibiotic therapy of meningitis induced by Streptococcus pneumoniae.Methods A total of 46 SD rats,3-week old,either male or female,received 10%chloral hydrate at the concentration（0.15- 0.30）mL/100 g by intraperitoneal injection.Among them,34 rats were established the brain damage in pneumococcal meningitis model successfully,which were divided into infection group（n= 20） and treatment group（n= 14）.The rest rats were included into control group（n= 12）.There was no significant difference of average weight and age among three group（P0.05）.①All of them underwent cisternal puncture. Infection group and treatment group received an equivalent volume of a Streptococcus pneumoniae suspension and control group received sterile saline as a placebo.The expression of neurotrophin-3 in the brain were observed（×400）.The rats were sacrificed at 24 h or 5 d post-infection and cortex were harvested（24 h infection group and 5 d infection group）.The expressions of neurotrophin-3 protein were detected by immunohistochemical staining methods in cerebral cortex and hippocampus.②Infection group were further divided into two sub-groups.Antibiotic treatment group（n = 8） received subcutaneous injection of ceftriaxone treatment for 3 days,and antibiotic control group（n = 6） received no treatment.③Control group were sacrificed immediately after 24 hours,5 days after receiving saline（24 h control group and 5 d control group）.Results In cerebral cortex and hippocampus,the expression of neurotrophin-3 was declined obviously at 24 h infection group and then restored in 5 d infection group,compared to control group（P0.01,P0.05）.The expression of neurotrophin-3 protein in antibiotic treatment group was lower than that of antibiotic control group（P0.05）.Conclusion The expression of neurotrophin-3 declined in the brain of rats after meningitis induced by Streptococcus pneumoniae.Neurotrophin-3
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2010年第5期333-336,共4页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
