摘要
目的通过研究人类结肠癌发生发展过程中新生淋巴管的发生分布特点,探讨新生淋巴管在结肠癌发展过程中的变化。方法采用鼠抗人D2-40单克隆抗体标记淋巴管内皮,通过光学显微镜观察新生淋巴管的数量和分布,探讨新生淋巴管与结肠癌发展的相关性。结果结肠低级别上皮内瘤变、高级别上皮内瘤变、结肠癌的黏膜固有层内均发现D2-40标记的新生毛细淋巴管,正常结肠壁、结肠低级别上皮内瘤变、结肠高级别上皮内瘤、结肠癌的新生毛细淋巴管数量分别为(7.93±2.36)个/HP、(33.14±11.96)个/HP、(65.40±23.13)个/HP及(42.20±17.20)个/HP。组间样本均数比较,差异有统计学意义(P<0.01)。结论在结肠癌的发生发展过程中,新生淋巴管数量在高级别上皮内瘤变组、结肠癌组、低级别上皮内瘤变组、正常结肠组织中呈递减趋势。在腺瘤癌变阶段淋巴管新生数量增多,提示新生的毛细淋巴管可作为腺瘤恶变的早期预测指标。
Objective To study the changes development process of lymphangiogenesis in colon carcinoma by studying the process of lymphangiogenesis in the occurrence and distribution characteristics in colon cancer.Methods Mark up the lymphatic vessels by using the mouse anti-human D2-40 monoclonal antibody marker and observed the number and distribution of new lymphatic vessels by the optical microscope,and analyzed the relationship between the changes of lymphangiogenesis and the development of colon carcinoma.Results D2-40 mark new lymphatic capillaries were found in colon low grade intraepithelial neoplasia,high grade intraepithelial neoplasia and colon mucosal lamina propria.The number of new lymphatic capillaries of normal colonic wall,colonic low grade intraepithelial neoplasia,colon high-level intraepithelial neoplasia and colon cancer the number of new lymphatic capillaries were（7.93±2.36）/HP,（33.14±11.96）/HP,（65.40±23.13）/HP and（42.20±17.20）/HP,respectively,the differences among four groups were significant（P0.01）.Conclusion In the course of the development of colon carcinoma,the number of new lymphatic vessels in high-grade intraepithelial neoplasia group,carcinoma of colon group,low-grade intraepithelial neoplasia,normal colon tissue show a decreasing trend.An increasing number of new lymphatic vessels are found in the stage of high-grade intraepithelial neoplasia,which suggests that lymphangiogenesis can be used as an early indicator of malignant transformation of adenoma.
出处
《广西医学》
CAS
2010年第9期1025-1027,共3页
Guangxi Medical Journal
基金
广西研究生教育创新计划项目(2009105981001M192)
