摘要
采用离子交换法,利用弱碱性阴离子交换树脂D315吸附小麦粉初提液中的α-淀粉酶抑制剂,对其静态吸附以及洗杂洗脱条件进行研究。通过对静态吸附条件的摸索,得出静态下的最佳工艺条件：上样料液的蛋白质量浓度ρ0=2.5-3.5 mg/mL、pH=8.5-9.5、温度t=30℃、转速150 r/min。最佳洗脱条件：0.1 mol/L NaCl洗杂,0.5mol/L NaCl洗脱。在该条件下,α-淀粉酶抑制剂纯化倍数为4.25倍,收率为64.58%。
The α-amylase inhibitor from wheat flour was separated by anion exchange resin D315.The conditions of its static adsorption,elution and washing were investigated.By static adsorption,the optimum conditions were obtained： protein concentration 2.5 mg/mL to 3.5 mg/mL,pH 8.5 to 9.5,temperature 30 ℃,rotation speed 150 r/min.The optimal conditions of the elution were as follows： 0.1 mol/L NaCl in the process of the washing impurity,0.5 mol/L NaCl in the process of elution.The purification reached up to 4.25 times and the recovery rate was 64.58%.
出处
《生物加工过程》
CAS
CSCD
2010年第5期44-47,共4页
Chinese Journal of Bioprocess Engineering
基金
国家重点基础研究发展计划(973计划)资助项目(2007CB714305)
关键词
Α-淀粉酶抑制剂
洗杂洗脱
离子交换法
静态吸附
α-amylase inhibitor
elution and washing
ion-exchange method
static adsorption
